Purification and characterization of pregnancy associated plasma protein A (PAPP-A)

Archives of Gynecology
P Bischof

Abstract

Pregnancy Associated Plasma Protein A (PAPP-A) has been isolated from late pregnancy plasma using ammonium sulphate precipitation, ion exchange chromatography, affinity chromatography on Concanavalin-A, gel filtration and negative affinity chromatography. It was found that PAPP-A is an alpha 2-glycoprotein of 750-820 000 MW, probably a dimer with each monomer being composed of 2 polypeptide chains of 218 000 MW. The amino acid composition as well as other physicochemical characteristics are similar to human alpha 2-macroglobulin. PAPP-A exhibits in vitro an inhibition of the activity of the complement system, of the caseinolytic activity of plasmin and possibly of the urokinase activation of plasminogen. The hypothesis that PAPP-A plays a role in the regulation of fibrinolysis during pregnancy is put forward.

References

Aug 1, 1976·Clinical Science and Molecular Medicine·G P CrawfordA S Douglas
Nov 1, 1979·British Journal of Obstetrics and Gynaecology·R SmithA Klopper
Jul 16, 1979·Clinica Chimica Acta; International Journal of Clinical Chemistry·P BischofA Klopper
Feb 15, 1976·American Journal of Obstetrics and Gynecology·T M LinS Gall
Jan 15, 1974·American Journal of Obstetrics and Gynecology·T M LinS Gall
Jul 1, 1974·Analytical Biochemistry·B PenkeK Kovács
Sep 1, 1974·The Journal of Clinical Investigation·T M LinW N Spellacy
Mar 1, 1972·The Biochemical Journal·J M JonesR A Kekwick
Sep 1, 1969·Clinica Chimica Acta; International Journal of Clinical Chemistry·S F Contractor, B Shane
Oct 1, 1952·Archives of Biochemistry and Biophysics·T ASTRUP, S MULLERTZ
May 1, 1954·The Biochemical Journal·E SCHRAME J BIGWOOD

❮ Previous
Next ❯

Citations

Nov 15, 1983·Clinica Chimica Acta; International Journal of Clinical Chemistry·N A Bersinger
Jul 31, 1995·Clinica Chimica Acta; International Journal of Clinical Chemistry·N A ZorinN N Semenkov
Jan 1, 1984·American Journal of Obstetrics and Gynecology·P BischofP C Sizonenko
Mar 1, 1988·American Journal of Obstetrics and Gynecology·K de BoerP E Treffers
Dec 1, 1994·The Journal of Allergy and Clinical Immunology·P Popken-HarrisG J Gleich
Sep 1, 1985·International Journal of Gynaecology and Obstetrics : the Official Organ of the International Federation of Gynaecology and Obstetrics·T YoshimuraM Maeyama
Aug 1, 1981·Australian and New Zealand Journal of Medicine·M J SinosichJ G Grudzinskas
Nov 1, 1980·American Journal of Obstetrics and Gynecology·P BischofA Klopper
Nov 1, 1983·The Journal of Experimental Zoology·F W Bazer, R M Roberts
Jun 1, 1986·British Journal of Obstetrics and Gynaecology·P BischofP C Sizonenko
May 1, 1983·British Journal of Obstetrics and Gynaecology·F H TsakokJ G Grudzinskas
Oct 1, 1981·British Journal of Obstetrics and Gynaecology·P BischofP C Sizonenko
Apr 1, 1986·American Journal of Reproductive Immunology and Microbiology : AJRIM·P Bischof, L Tseng
Jul 1, 1983·Annals of Clinical Biochemistry·D R Pledger, A Belfield

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.