PMID: 3096734Dec 1, 1986Paper

Purification and characterization of propylamine transferase from Sulfolobus solfataricus, an extreme thermophilic archaebacterium

European Journal of Biochemistry
G CacciapuotiV Zappia

Abstract

The enzyme propylamine transferase, catalyzing the transfer of the propylamine moiety from S-adenosyl(5')-3-methylthiopropylamine to several amine acceptors, has been purified 643-fold in 20% yield from Sulfolobus solfataricus, an extreme thermophilic archaebacterium optimally growing at 87 degrees C. The purified enzyme (specific activity 2.05 units/mg protein), is homogeneous by criteria of gel electrophoresis, gel filtration, isoelectric focusing and ultracentrifugation analysis. The molecular mass of the native enzyme was estimated to be about 110 kDa by gel permeation and ultracentrifugation analysis. The protein migrates on SDS/polyacrylamide gel electrophoresis as a single band of 35 kDa, suggesting that the enzyme is a trimer composed by identical subunits. An optimum pH of 7.5 and an acidic isoelectric point of 5.3 have been calculated. The optimum temperature was 90 degrees C and no loss of activity is observable even after exposure of the purified enzyme to 100 degrees C for 1 h. No reducing agents are required for enzymatic activity. Substrate specificity towards the amine acceptors is rather broad in that 1,3-diaminopropane (Km = 1675 microM), putrescine (Km = 3850 microM), sym-norspermidine (Km = 954 microM) and s...Continue Reading

References

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Citations

Jan 9, 2013·Extremophiles : Life Under Extreme Conditions·D EsserB Siebers
Jul 25, 2008·The Journal of Biological Chemistry·Teresa N Giles, David E Graham
Nov 1, 1990·Plant Physiology·G D KuehnG C Phillips
May 1, 2002·The Journal of Biological Chemistry·David E GrahamRobert H White
Jun 9, 2016·The Journal of Biological Chemistry·Anthony J Michael

Related Concepts

Archaea
Molecular Sieve Chromatography
High Pressure Liquid Chromatography Procedure
SDS-PAGE
Enzyme Stability
Hydrogen-Ion Concentration
Isoelectric Focusing
Spermidine Synthase
Substrate Specificity
Transferase

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