Purification and characterization of proteasomes from Saccharomyces cerevisiae

Current Protocols in Protein Science
M H Glickman, O Coux

Abstract

The proteasome plays a central role in eukaryotic cells since it is responsible for the degradation of specific proteins involved in a large range of cellular processes. Analysis of proteasome mechanisms of action, or in vitro reconstitution, or dissection of the complex biological pathways in which it partakes, requires a reliable source of pure active proteasome. Although the biologically relevant form of the proteasome is usually considered to be the 26S proteasome, this unit describes different methods for purification and study of both 26S and 20S proteasomes from Saccharomyces cerevisiae cells.

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Citations

Feb 4, 2011·Molecular Biology of the Cell·Anca F SavulescuAmnon Harel
Jul 7, 2014·Nature Chemical Biology·Daria KrutauzMichael H Glickman
Jun 11, 2015·Current Protocols in Cell Biology·Yanjie LiMark Hochstrasser
Apr 30, 2016·The Journal of Biological Chemistry·Vanessa WelkSilke Meiners
Dec 19, 2020·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Vandita DwivediMichal Sharon
Nov 20, 2008·Protein Expression and Purification·Aurélie Y Le FeuvreOlivier Coux
Nov 16, 2021·Future Medicinal Chemistry·Haili LinMingdong Huang

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