Nov 1, 1975

Purification and characterization of proteinase inhibitors from adzuki beans (Phaseolus angularis)

Journal of Biochemistry
C Yoshida, M Yoshikawa


Two proteinase inhibitors, designated as inhibitors I and II, were purified from adzuki beans (Phaseolus angularis) by chromatographies on DEAE- and CM-cellulose, and gel filtration on a Sephadex G-100 column. Each inhibitor shows unique inhibitory activities. Inhibitor I was a powerful inhibitor of trypsin [EC], but essentially not of chymotrypsin ]EC]. On the other hand, inhibitor II inhibited chymotrypsin more strongly than trypsin. The molecular weights estimated from the enzyme inhibition were 3,750 and 9,700 for inhibitors I and II, respectively, assuming that the inhibitions were stoichiometric and in 1 : 1 molar ratio. The amino acid compositions of both inhibitors closely resemble those of low molecular weight inhibitors of other leguminous seeds: they contain large amounts of half-cystine, aspartic acid and serine, and little or no hydrophobic and aromatic amino acids. Inhibitor I lacks both tyrosine and tryptophan residues. The molecular weights were calculated to be 7,894 and 8,620 for inhibitors I and II, respectively. The reliability of these molecular weights was confirmed by the sedimentation equilibrium and 6 M guanidine gel filtration methods. On comparison with the values obtained from enzym...Continue Reading

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Mentioned in this Paper

Trypsin Inhibitors
Chromatography, DEAE-Cellulose
Plasma Protein Binding Capacity
Enzyme Inhibitors
Molecular Sieve Chromatography
Hydrogen-Ion Concentration

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