PMID: 6169716Sep 25, 1981Paper

Purification and characterization of recombinant human leukocyte interferon (IFLrA) with monoclonal antibodies.

The Journal of Biological Chemistry
T StaehelinS Pestka

Abstract

Recombinant human leukocyte interferon produced in bacteria (IFLrA) was purified to homogeneity with the use of monoclonal antibodies against leukocyte interferon. The purified interferon exhibited a single band of Mr = approximately 19,000 on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Amino acid analysis and the NH2-terminal sequence were consistent with the sequence predicted from the DNA. Some of the purified product contained NH2-terminal methionine; the terminal methionine was removed from the rest of the chains.

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