Purification and Characterization of Schwann Cells from Adult Human Skin and Nerve

ENeuro
Jo Anne StrattonJ Biernaskie

Abstract

Despite its modest capacity for regeneration, peripheral nervous system injury often results in significant long-term disability. Supplementing peripheral nervous system injury with autologous Schwann cells (SCs) may serve to rejuvenate the postinjury environment to enhance regeneration and ultimately improve functional outcomes. However, human nerve-derived SC (hN-SC) collection procedures require invasive surgical resection. Here, we describe the characterization of SCs from adult human skin (hSk-SCs) of four male donors ranging between 27 and 46 years old. Within five weeks of isolating and culturing adherent mixed skin cells, we were able to obtain 3-5 million purified SCs. We found that hSk-SCs appeared transcriptionally indistinguishable from hN-SCs with both populations exhibiting expression of SC genes including: SOX10, SOX9, AP2A1, CDH19, EGR1, ETV5, PAX3, SOX2, CX32, DHH, NECL4, NFATC4, POU3F1, S100B, and YY1. Phenotypic analysis of hSk-SCs and hN-SCs cultures revealed highly enriched populations of SCs indicated by the high percentage of NES+ve, SOX10+ve, s100+ve and p75+ve cells, as well as the expression of a battery of other SC-associated proteins (PAX3, CDH19, ETV5, SOX2, POU3F1, S100B, EGR2, and YY1). We further...Continue Reading

Citations

Dec 29, 2018·The Journal of Comparative Neurology·Lynn NazarethJenny A K Ekberg
Apr 4, 2018·Lasers in Medical Science·Luana Gabriel de SouzaRafael Inácio Barbosa
Apr 11, 2021·Cell and Tissue Banking·Jiahong YuJiabo Hu
Jul 6, 2021·Frontiers in Cellular Neuroscience·Paula V MonjeXiao-Ming Xu
Oct 2, 2021·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Peter Arthur-Farraj, Michael P Coleman
Jan 7, 2022·Journal of Anatomy·Samuele NegroMichela Rigoni

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Methods Mentioned

BETA
PCR
FACS

Key Resources (RRID) Mentioned

AB_823672
AB_2286684
AB_10013383
AB_2184560
AB_2314858
AB_2636922
AB_2313561
AB_2268536
AB_2313773
AB_10854564

Software Mentioned

GraphPad Prism
ImageJ
nCounter
ImageExpress
iTEM
Tapestation

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