PMID: 2506013Aug 15, 1989Paper

Purification and characterization of subforms of the guanine-nucleotide-binding proteins G alpha i and G alpha o

European Journal of Biochemistry
J Lang

Abstract

Five different pertussis-toxin-sensitive guanine-nucleotide-binding proteins (G proteins) were purified from bovine brain. Immunochemical characterization of alpha subunits identified two G alpha o proteins (G alpha o-I and G alpha o-II), two 41-kDa G alpha i proteins (G alpha i-I and G alpha i-II) and the 40-kDa G alpha i2 protein. Site-directed antisera specific for G alpha o proteins did not differentiate between G alpha o-I and G alpha o-II. However, in situ peptide mapping using polyacrylamide gel electrophoresis revealed distinct cleavage products with different proteases for each of these proteins. Additionally comparison of Rf values demonstrated a slightly faster migration for G alpha o-II than for G alpha o-I, which is the only type of G alpha o protein present in cell membranes of the neuroblastoma/glioma cell line NG 108-15. The importance of these structural differences and possible functional implications are discussed.

References

Jan 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·S M MumbyA G Gilman
May 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·K P Van MeursM Vaughan
Aug 14, 1987·Biochemical and Biophysical Research Communications·A M SchultzM Vaughan
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May 12, 1988·Nature·E J Neer, D E Clapham
May 16, 1988·Biochemical and Biophysical Research Communications·P BrabetV Homburger

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Citations

Jul 1, 1991·Journal of Neurochemistry·J N LaroccaR W Ledeen
Feb 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·T ExnerB Nürnberg
May 10, 1995·Annals of the New York Academy of Sciences·P SokoloffJ C Schwartz
Mar 15, 1991·Biochemical and Biophysical Research Communications·K SpicherW Rosenthal

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