PMID: 6104509May 27, 1980Paper

Purification and characterization of the amino-terminal propeptide of Pro alpha 1(I) chains from embryonic chick tendon procollagen

Biochemistry
D M PesciottaB R Olsen

Abstract

A peptide with an apparent molecular weight of 23,000 was isolated from the medium of cultured chick embryo tendons. Comparison of tryptic peptides derived from the medium peptide and from the amino-terminal, bacterial collagenase resistant portion of Type I procollagen indicated that the medium peptide represented the amino-terminal precursor-specific region of the pro alpha 1 (I) chain of procollagen. This conclusion was supported by the demonstration that antibodies against the medium peptide reacted with Type I procollagen in a radioimmune assay but did not react with a peptide derived from the carboxy-terminal propeptide of Type I procollagen. In addition, the reaction with Type I procollagen was inhibited with the purified amino-terminal, collagenase-resistant portion of pro alpha 1 (I) chains. Finally, amino acid sequencing demonstrated that the amino propeptide of dermatosparactic calf pN alpha 1 (I) chains and the medium peptide have similar amino-terminal sequences. Carbohydrate analysis established the presence of one residue of N-acetylglucosamine and a trace of mannose and galactose.

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Citations

Jan 1, 1987·Cell and Tissue Research·R FleischmajerB R Olsen
May 1, 1996·In Vitro Cellular & Developmental Biology. Animal·D M PetersT Compton
Jun 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·R FleischmajerO Lovelace
Aug 1, 1983·The Journal of Cell Biology·L Pollack, P H Atkinson
Jul 15, 1983·European Journal of Biochemistry·H G FoellmerL Tuderman
Mar 1, 1993·Matrix : Collagen and Related Research·M WarmanB R Olsen
Jan 1, 1985·Annals of the New York Academy of Sciences·F RamirezM Sobel
Mar 1, 1983·Biochemistry·S GerardW M Mitchell
Aug 1, 1983·Seminars in Arthritis and Rheumatism·M E Nimni
Aug 1, 1987·The Journal of Investigative Dermatology·R FleischmajerB R Olsen

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