PMID: 39896Aug 1, 1979

Purification and characterization of the CFA/I antigen of enterotoxigenic Escherichia coli

Infection and Immunity
D G EvansJ A Pauley


The fimbral colonization factor antigen CFA/I of enterotoxigenic Escherichia coli was purified and characterized. The initial purification step was release of these fimbriae from the bacterial cells by homogenization with a Waring blender. Common fimbriae and flagellar antigen were avoided by careful control of growth conditions and the use of a nonmotile (H-) mutant of the prototype strain H-10407 (O78:H11). The essential purification steps were membrane filtration (Millipore Corp.), ammonium sulfate fractionation, and negative diethylaminoethyl-Sephadex column chromatography. Yields were approximately 4.0 mg of CFA/I protein per g (wet weight) of bacteria. Purified CFA/I is a fimbrial molecule 7.0 nm in diameter and has an average molecular weight of 1.6 X 10(6), as determined by sedimentation equilibrium. CFA/I is a polymer of identical subunits of molecular weight 23,800 with an N-terminal valine, 37% hydrophobic amino acid residues, and 11 residues of proline per mol. The purified antigen retains its morphology, antigenicity, and biological activity. Purified antigen retains its morphology, antigenicity, and biological activity. Purified CFA/I exhibits mannose-resistant hemagglutination of human group A, bovine, and chicke...Continue Reading


Jan 1, 1981·Critical Reviews in Microbiology·J W CostertonK J Cheng
Jan 1, 1983·Critical Reviews in Microbiology·G W Jones, R E Isaacson
Jan 1, 1981·Transactions of the Royal Society of Tropical Medicine and Hygiene·H L Du Pont
Apr 3, 1998·Microbiology and Immunology·T TamamotoT Yamashiro
Feb 26, 2000·Journal of Travel Medicine·P ParedesH L DuPont
Dec 14, 2005·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Stefan LüdiMichael H Stoffel
Mar 30, 2010·Molecular Microbiology·Veronika TchesnokovaStephen J Savarino
May 9, 2012·Physical Biology·Ling CaoXinghong Yang
Jan 1, 1990·Urological Research·T MatsumotoJ Kumazawa
Jan 1, 1990·Microbiology and Immunology·Y Honma, N Nakasone
Jan 1, 1980·Infection·B Sugarman
Sep 1, 1995·Journal of Industrial Microbiology·F J Cassels, M K Wolf
Jan 5, 2012·The Journal of Biological Chemistry·Sujay ChattopadhyayStephen J Savarino
Jan 1, 1990·Microbiology and Immunology·A HokamaN Nakasone
Oct 27, 1999·Proceedings of the National Academy of Sciences of the United States of America·H SakellarisJ R Scott
May 29, 1999·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·A M AlvesL C Ferreira
Jan 25, 2002·Journal of Bacteriology·Thomas G DuthyMichael W Heuzenroeder
Jan 15, 1994·FEMS Microbiology Letters·T YamashiroM Iwanaga
Mar 27, 1999·Molecular Microbiology·H Sakellaris, J R Scott
May 23, 2012·Canadian Journal of Microbiology·Kathrin Kuehni-BoghenborMichael H Stoffel
Jan 1, 1988·Scandinavian Journal of Infectious Diseases·M JertbornS Iwarson
Jan 1, 1987·Microbiology and Immunology·K IkeM Kashiwazaki
May 19, 2000·Infection and Immunity·M PichelG Viboud
Dec 8, 2007·Microbiology and Molecular Biology Reviews : MMBR·Sean-Paul Nuccio, Andreas J Bäumler

Related Concepts

Hemagglutination Inhibition Tests
Synapsin I
Alkalescens-Dispar Group
Squamous Transitional Epithelial Cell Count
Science of Morphology
Small Intestinal Wall Tissue
Bos taurus

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Synapse Loss as Therapeutic Target in MS

As we age, the number of synapses present in the human brain starts to decline, but in neurodegenerative diseases this occurs at an accelerated rate. In MS, it has been shown that there is a reduction in synaptic density, which presents a potential target for treatment. Here is the latest research on synapse loss as a therapeutic target in MS.

Artificial Intelligence in Cardiac Imaging

Artificial intelligence (ai) techniques are increasingly applied to cardiovascular (cv) medicine in cardiac imaging analysis. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

Social Learning

Social learning involves learning new behaviors through observation, imitation and modeling. Follow this feed to stay up to date on the latest research.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Single Cell Chromatin Profiling

Techniques like ATAC-seq and CUT&Tag have the potential to allow single cell profiling of chromatin accessibility, histones, and TFs. This will provide novel insight into cellular heterogeneity and cell states. Discover the latest research on single cell chromatin profiling here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.