PMID: 8960116Aug 1, 1996Paper

Purification and characterization of the eighth and ninth components of carp complement

Molecular Immunology
T UemuraM Nakao

Abstract

Complement components corresponding to mammalian C8 and C9 were isolated from carp (Cyprinus carpio) serum. Carp C8 (M(r) 146,000) proved to be a gamma-globulin composed of three polypeptide chains (alpha-chain, M(r) 62,000; beta-chain, M(r) 62,000; gamma-chain, M(r) 22,000). The alpha-chain was disulfide-linked to the gamma-chain and the beta-chain was non-covalently associated with the alpha-gamma chain, in fair agreement with mammalian C8. However, the N-terminal amino acid sequences of the three subunits showed no homology with those of human C8. Carp C9 was an alpha-globulin composed of a single polypeptide (M(r) 91,000) and the N-terminus was blocked. Carp serum depleted of C8 did not hemolyse either carp antibody-sensitized sheep erythrocytes or non-sensitized rabbit erythrocytes, while C9-depleted carp serum did not hemolyse the former, but did hemolyse the latter target cells, as in the case of C9-depleted human serum.

References

Sep 1, 1991·Immunology Today·T Kinoshita
Jan 1, 1985·Human Genetics·S RogdeB Olaisen
Jan 1, 1985·Developmental and Comparative Immunology·C W Vogel, H J Müller-Eberhard
Dec 1, 1973·The Journal of Comparative Neurology·S O Ebbesson, C B Campbell
Jan 1, 1969·Journal of Chemical Education·J M Brewer, R B Ashworth
Jan 1, 1984·Springer Seminars in Immunopathology·H J Müller-Eberhard
Nov 1, 1981·Molecular Immunology·H Kitamura, K Nagaki
Jan 1, 1993·Developmental and Comparative Immunology·S TomlinsonA F Esser

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Citations

Aug 10, 2006·Immunogenetics·Masaru Nonaka, Ayuko Kimura
Oct 17, 2001·Developmental and Comparative Immunology·B G Magor, K E Magor
Oct 17, 2001·Developmental and Comparative Immunology·I K ZarkadisJ D Lambris
Feb 20, 2003·Developmental and Comparative Immunology·Alexandra KazantziIoannis K Zarkadis
Jan 23, 1999·Immunological Reviews·J O Sunyer, J D Lambris
Nov 8, 2005·Molecular Immunology·Maria P ChondrouIoannis K Zarkadis
Jul 17, 2012·Fish & Shellfish Immunology·Wickramaarachchige Don Niroshana WickramaarachchiJehee Lee
Aug 27, 2002·Fish & Shellfish Immunology·M Claire H Holland, John D Lambris
Mar 19, 2011·Developmental and Comparative Immunology·Miki NakaoTomonori Somamoto

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