PMID: 9530955Apr 8, 1998Paper

Purification and characterization of the MUC1 mucin-type glycoprotein, epitectin, from human urine: structures of the major oligosaccharide alditols

Glycoconjugate Journal
V P BhavanandanB Fournet

Abstract

The MUC1 glycoprotein, epitectin, a component of the human bladder epithelium, was purified from human urine. Sedimentation equilibrium analysis and gel filtration using polysaccharide or protein standards revealed a polydisperse preparation with molecular weights ranging from about 0.9 to 1.3 x 10(6). This suggests that in the native state epitectin exists as aggregates of three or four monomer units of 350-400 kDa. Epitectin was found to have significant affinity to hexyl-, octyl- or phenyl agarose indicating that hydrophobic interactions and possibly carbohydrate-carbohydrate interactions may be responsible for the self-association. Chemical and enzymic deglycosylation of [125I]-labeled urine epitectin and metabolically labeled H.Ep.2 epitectin resulted in extremely polydisperse products. The buoyant densities of epitectin purified from urine and H.Ep.2 cells were found to be 1.39-1.40 g ml(-1), suggesting that the total carbohydrate content of these preparations is not significantly different. The O-linked saccharides of epitectin were fractionated by HPLC and analyzed by permethylation and FAB-MS. The neutral saccharides from both sources contain three common structures, namely Gal1 --> 3GalNAc, GlcNAc1 --> 6 (Gal1 --> 3) ...Continue Reading

Citations

Sep 18, 1998·Electrophoresis·N H Packer, M J Harrison
Apr 20, 2001·Biological Chemistry·F G Hanisch
Nov 2, 2016·Microscopy Research and Technique·Maria MastrodonatoGiovanni Scillitani
Nov 15, 2014·Glycoconjugate Journal·Iwona Kątnik-PrastowskaAgata Matejuk
Mar 7, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Erik P LillehojK Chul Kim
Dec 25, 2007·Carbohydrate Research·Sergey Y VakhrushevJasna Peter-Katalinić

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