PMID: 7540499Mar 1, 1995Paper

Purification and characterization of the serotype-specific polysaccharide antigen of Trichosporon cutaneum serotype II: a disease-related antigen of Japanese summer-type hypersensitivity pneumonitis

Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology
T MizobeA Misaki

Abstract

Summer-type hypersensitivity pneumonitis (SHP) is a unique type of hypersensitivity pneumonitis and the most prevalent in Japan. Our previous study clarified that the causative agent of the disease is Trichosporon cutaneum, and that the patients with SHP have high titres of antibodies against the serotype-specific antigen of polysaccharide nature which exist in the high molecular weight fraction of the culture supernatant of the yeast. In this study, we purified the serotype-specific antigen of serotype II T. cutaneum by gel filtration and affinity chromatography using a monoclonal antibody, D-8, specific for a high molecular weight antigen of serotype II T. cutaneum, and elucidated the structure of the antigen. This affinity-purified antigen was shown to be an essentially acidic polysaccharide comprising mannose, xylose, and glucuronic acid (6:44:4.7). Chemical analysis showed that this polysaccharide antigen contains a (1-3)-linked mannan backbone attached with short side chains of (1-4)-linked mannose and a small proportion of (1-2)-linked xylose residues by substituting the 2- or 4-positions of the (1-3)-linked mannose residues of the main chain. Approximately one-fifth of the side chains were terminated with glucuronic aci...Continue Reading

References

Aug 1, 1979·Carbohydrate Research·A K BhattacharjeeC P Glaudemans
Sep 1, 1978·Immunochemistry·A K BhattacharjeeC P Glaudemans
Jan 1, 1988·Chest·H K Gold, R C Leinbach
Feb 1, 1988·The Journal of Allergy and Clinical Immunology·K YoshidaS Araki
Aug 1, 1973·Analytical Biochemistry·N Blumenkrantz, G Asboe-Hansen
Apr 25, 1966·Biochimica Et Biophysica Acta·A Blandamer, I Danishefsky
Jul 1, 1984·The Journal of Allergy and Clinical Immunology·J N Fink
Mar 1, 1984·Chest·R D MarksT R Martin
Apr 1, 1982·Proceedings of the Society for Experimental Biology and Medicine·I LeeH Friedman

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Citations

Oct 9, 2001·European Journal of Biochemistry·T IchikawaT Shinoda
Oct 7, 2011·Clinical Microbiology Reviews·Arnaldo L ColomboGuilherme M Chaves
Mar 11, 2000·American Journal of Respiratory and Critical Care Medicine·T MiyagawaN Tanigawa
Dec 21, 2002·American Journal of Respiratory and Critical Care Medicine·Yasujiro MatsunagaYasuyuki Yoshizawa
Jun 24, 2008·Mycopathologia·Thomas C Chagas-NetoArnaldo L Colombo
Jul 25, 2020·American Journal of Respiratory and Critical Care Medicine·Ganesh RaghuKevin C Wilson

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