PMID: 6170938Sep 25, 1981Paper

Purification and characterization of the messenger RNA for the heavy chain of rat immunoglobulin E

Nucleic Acids Research
M E Zajdel-BlairH Bennich

Abstract

The isolation and translational properties of rat immunoglobulin E (IgE) heavy chain mRNA are described. The mRNA has a sedimentation coefficient of approximately 18S, a chain length of about 2000 nucleotides and directs the synthesis in vitro of a polypeptide of 65000 molecular weight in an mRNA-dependent rabbit reticulocyte lysate. Inclusion of dog pancreatic microsomes in the cell-free translation system resulted in a heavy chain product of about 75000 molecular weight, presumably as a consequence of glycosylation in vitro. This species co-migrated in an SDS polyacrylamide gel with mature IgE heavy chain. Substantial purification of heavy chain mRNA was achieved by denaturing sucrose gradient centrifugation and agarose gel electrophoresis.

References

Sep 1, 1979·The Journal of General Virology·P BoulangerW C Russell
Jan 1, 1978·Immunological Reviews·K J Dorrington, H H Bennich
Aug 1, 1976·European Journal of Biochemistry·H R Pelham, R J Jackson
Jan 1, 1977·The Journal of General Virology·W C Russell, G E Blair
Jan 1, 1976·Analytical Biochemistry·J M Bailey, N Davidson
Jun 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·H Aviv, P Leder
Jul 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·C W AndersonR F Gesteland
Jul 1, 1974·European Journal of Biochemistry·W M Bonner, R A Laskey

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