PMID: 2512124Nov 20, 1989Paper

Purification and characterization of three (1----4)-beta-D-xylan endohydrolases from germinated barley

European Journal of Biochemistry
A M SladeG B Fincher

Abstract

Three (1----4)-beta-D-xylan xylanohydrolases (xylan endohydrolases, EC 3.2.1.8) have been purified 1200-2800-fold from extracts of germinated barley (Hordeum vulgare L. cv. Clipper) by a sequence of ammonium sulphate fractionation, Procion-blue-dye chromatography, ion-exchange and gel filtration chromatography. The enzymes are likely to function in the depolymerization of cell wall arabinoxylans during mobilization of the starchy endosperm. They are classified as endohydrolases on the basis of analyses of products released during hydrolysis of a (1----4)-beta-xylan. The three xylan endohydrolases are monomeric proteins of apparent Mr 41,000 and all have isoelectric points of 5.2. The sequences of the 30 NH2-terminal amino acids of the three enzymes are the same, but it is not yet known whether they represent the products of separate genes or originate by differences in post-translational modification of a single gene product.

References

May 1, 1977·Analytical Biochemistry·J J Sedmak, S E Grossberg
Jan 1, 1982·European Journal of Biochemistry·J R Woodward, G B Fincher
Apr 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·G B FincherK K Thomsen
Nov 1, 1986·Planta·E Benjavongkulchai, M S Spencer

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Citations

Feb 12, 2004·Biochimica Et Biophysica Acta·Kurt GebruersJan A Delcour
Aug 1, 1997·International Journal of Biological Macromolecules·M HrmovaG B Fincher
Jun 8, 2001·The Plant Journal : for Cell and Molecular Biology·M P CaspersV Cameron-Mills
Sep 10, 2013·Food Microbiology·Sofie MalflietGuido Aerts
Mar 24, 2016·Phytopathology·Bode OlukoluPeter John Balint-Kurti
Mar 27, 2007·Biochemical and Biophysical Research Communications·Steven Van CampenhoutGuido Volckaert
Feb 11, 2016·Biochemical Society Transactions·Vasilios M E AndriotisRobert A Field
May 27, 2021·Plant Science : an International Journal of Experimental Plant Biology·Natalie S BettsVincent Bulone

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