PMID: 9173903Apr 1, 1997Paper

Purification and characterization of three constitutive cytochrome P-450 isoforms from bovine olfactory epithelium

The Biochemical Journal
V LongoP G Gervasi

Abstract

Three constitutive forms of cytochrome P-450 (P-450s) were isolated from olfactory microsomes of cattle. The purified P-450s, designated P-450bov1, P-450bov2 and P-450bov3, were electrophoretically nearly homogeneous by SDS/PAGE and their apparent relative molecular masses were estimated to be 50000, 53000 and 51000 respectively. As indicated by several criteria including the N-terminal sequence and absorption spectra, the three olfactory forms of P-450 were distinct from each other and from all the other P-450s currently known in cattle. P-450bov1 and P-450bov2 were purified in the low-spin state, whereas P-450bov3 was in the high-spin state. Studies to evaluate, by Western blot analysis, the reactivity of these purified P-450s with antibodies raised against rat hepatic P-450 2E1, 2B, 1A and 3A and rabbit olfactory P-450NMa and P-450NMb showed that P-450bov3 strongly cross-reacted with anti-P-450NMb IgG, and P-450bov1 moderately with anti-P-450NMa IgG. As determined by immunoblots, P-450bov1 and P-450bov3 represented a great portion of the total olfactory P-450. In a reconstituted system with NADPH:cytochrome P-450 reductase and phospholipids, P-450bov1 was more active in the metabolism of xenobiotic compounds (i.e. O-de-ethyl...Continue Reading

Citations

Feb 1, 2005·The Journal of Comparative Neurology·Tun-Tzu YuTimothy S McClintock
Dec 16, 1998·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·S MariniP G Gervasi
Dec 30, 2010·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Yasuhiro UnoHiroshi Yamazaki
Jun 15, 2012·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Vanessa ZancanellaMauro Dacasto
Jun 12, 2003·Chemical Reviews·Chin-Chuan WeiDennis J Stuehr
Jun 3, 1999·Biochemical Pharmacology·S KoskelaH Raunio

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