PMID: 3770442Jul 1, 1986Paper

Purification and characterization of toad prolactin

General and Comparative Endocrinology
K YamamotoS Kikuyama

Abstract

Prolactin (PRL) was purified from anterior pituitary glands of adult toad (Bufo japonicus formosus) by extraction of acetone-dried pituitary powder with acid acetone and chromatography on DEAE-cellulose and Sephadex G-100. Purification was monitored by radioimmunoassay developed for bullfrog PRL. Toad PRL thus obtained is as potent as bovine PRL in promoting collagen synthesis in the bullfrog tadpole tail fin. HPLC of the toad PRL on a reverse-phase column shows that the preparation behaves as a homogeneous protein. The hormone has a molecular weight of 23,000 as determined by SDS gel electrophoresis. The isoelectric point is 5.5 as determined by analytical electrofocusing. The amino acid composition closely resembles that of bullfrog PRL.

References

Apr 1, 1976·The Journal of Endocrinology·P J KnightJ M Hamilton
Nov 1, 1975·General and Comparative Endocrinology·C G ScanesA Chadwick
Feb 1, 1979·International Journal of Peptide and Protein Research·H Kawauchi, M Tubokawa
Jan 1, 1977·General and Comparative Endocrinology·S W FarmerC H Li
Jan 1, 1976·International Journal of Peptide and Protein Research·C H Li
Nov 1, 1969·Endocrinology·S EllisP X Callahan
Aug 25, 1974·FEBS Letters·M Wallis
Dec 1, 1970·Archives of Biochemistry and Biophysics·C H LiY A Pankov
Apr 27, 1971·Biochimica Et Biophysica Acta·P Righetti, J W Drysdale
Mar 1, 1983·General and Comparative Endocrinology·H KawauchiY Nakai
Jan 1, 1984·General and Comparative Endocrinology·P Prunet, L M Houdebine
Oct 1, 1982·General and Comparative Endocrinology·H PapkoffM M Oosthuizen
Jan 1, 1983·Archives of Biochemistry and Biophysics·C H Li, D Chung
Feb 1, 1981·Endocrinologia Japonica·K Yamamoto, S Kikuyama
Apr 1, 1982·Endocrinologia Japonica·K Yamamoto, S Kikuyama
Mar 1, 1980·General and Comparative Endocrinology·W H Burke, H Papkoff
Jul 23, 1955·Nature·S M FITCHR D HARKNESS
Dec 28, 1964·Annals of the New York Academy of Sciences·B J DAVIS

❮ Previous
Next ❯

Citations

Feb 17, 2001·General and Comparative Endocrinology·N TakahashiS Kikuyama
Feb 1, 1988·General and Comparative Endocrinology·S Garcia-NavarroF Gracia-Navarro
Jan 1, 1990·General and Comparative Endocrinology·K MatsudaS Kikuyama
Sep 1, 1990·General and Comparative Endocrinology·C H ChengC C Wong
Apr 1, 1991·General and Comparative Endocrinology·S C BrownS Kikuyama
Apr 1, 1991·General and Comparative Endocrinology·T Kobayashi, S Kikuyama
Mar 1, 1989·General and Comparative Endocrinology·T KobayashiY Yokoo
Jun 1, 1989·General and Comparative Endocrinology·K YamamotoS Ishii
Jan 10, 2001·Canadian Journal of Physiology and Pharmacology·S KikuyamaA M Polzonetti-Magni

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2021 Meta ULC. All rights reserved