PMID: 6838843Mar 1, 1983

Purification and characterization of two forms of DNA polymerase alpha from HeLa cell nuclei

Biochemistry
T EnomotoM Yamada

Abstract

Isolated nuclei contained two active forms of DNA polymerase alpha (form I and form II). Form II was extracted from nuclei by KCl at concentrations lower than 0.18 M. Above 0.18 M selective extraction of form I was observed. The purified two active forms differed in chromatographic and electrophoretic behaviors, in their salt requirement for optimal activity, and in preference of template-primers, although both forms exhibited properties characteristic of DNA polymerase alpha such as sensitivity of N-ethylmaleimide, 1-beta-D-arabino-furanosylcytosine triphosphate, and aphidicolin. Marked difference between the two forms was preference of template-primer that form I was more active with poly(dT).(rA)10 than poly(dA).(dT)12 whereas form II exhibited higher activity with poly(dA).(dT)12 than poly(dT).(rA)10. Possible roles of two forms of DNA polymerase alpha in the processes of DNA replication will be discussed.

References

Jun 29, 1978·Biochemical and Biophysical Research Communications·M OhashiS Ikegami
Aug 1, 1978·European Journal of Biochemistry·C L Brakel, A B Blumenthal
Dec 1, 1978·FEBS Letters·F Bieri-Bonniot, A R Schuerch
Dec 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·G Brun, A Weissbach
Jan 1, 1977·Annual Review of Biochemistry·A Weissbach
Jul 2, 1976·Biochimica Et Biophysica Acta·S Seki, G C Mueller
Jan 2, 1975·European Journal of Biochemistry·H J Hachmann, A G Lezius
Jul 24, 1974·Biochimica Et Biophysica Acta·S YoshidaT Ando
Feb 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·J Shlomai, A Kornberg
Aug 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·P LamotheE Baril

Related Concepts

Cell Nucleus
DNA Polymerase II
DNA Polymerase N3
SDS-PAGE
HeLa Cells
Alloenzymes

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Synthetic Genetic Array Analysis

Synthetic genetic arrays allow the systematic examination of genetic interactions. Here is the latest research focusing on synthetic genetic arrays and their analyses.

Congenital Hyperinsulinism

Congenital hyperinsulinism is caused by genetic mutations resulting in excess insulin secretion from beta cells of the pancreas. Here is the latest research.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Epigenetic Memory

Epigenetic memory refers to the heritable genetic changes that are not explained by the DNA sequence. Find the latest research on epigenetic memory here.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Femoral Neoplasms

Femoral Neoplasms are bone tumors that arise in the femur. Discover the latest research on femoral neoplasms here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.