PMID: 6838843Mar 1, 1983

Purification and characterization of two forms of DNA polymerase alpha from HeLa cell nuclei

T EnomotoM Yamada


Isolated nuclei contained two active forms of DNA polymerase alpha (form I and form II). Form II was extracted from nuclei by KCl at concentrations lower than 0.18 M. Above 0.18 M selective extraction of form I was observed. The purified two active forms differed in chromatographic and electrophoretic behaviors, in their salt requirement for optimal activity, and in preference of template-primers, although both forms exhibited properties characteristic of DNA polymerase alpha such as sensitivity of N-ethylmaleimide, 1-beta-D-arabino-furanosylcytosine triphosphate, and aphidicolin. Marked difference between the two forms was preference of template-primer that form I was more active with poly(dT).(rA)10 than poly(dA).(dT)12 whereas form II exhibited higher activity with poly(dA).(dT)12 than poly(dT).(rA)10. Possible roles of two forms of DNA polymerase alpha in the processes of DNA replication will be discussed.


Jun 29, 1978·Biochemical and Biophysical Research Communications·M OhashiS Ikegami
Aug 1, 1978·European Journal of Biochemistry·C L Brakel, A B Blumenthal
Dec 1, 1978·FEBS Letters·F Bieri-Bonniot, A R Schuerch
Dec 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·G Brun, A Weissbach
Jan 1, 1977·Annual Review of Biochemistry·A Weissbach
Jul 2, 1976·Biochimica Et Biophysica Acta·S Seki, G C Mueller
Jan 2, 1975·European Journal of Biochemistry·H J Hachmann, A G Lezius
Jul 24, 1974·Biochimica Et Biophysica Acta·S YoshidaT Ando
Feb 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·J Shlomai, A Kornberg
Aug 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·P LamotheE Baril

Related Concepts

Cell Nucleus
DNA Polymerase II
DNA Polymerase N3
HeLa Cells

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