PMID: 11929278Apr 4, 2002Paper

Purification and characterization of two antifungal chitinases extracellularly produced by Bacillus amyloliquefaciens V656 in a shrimp and crab shell powder medium

Journal of Agricultural and Food Chemistry
San-Lang WangChi-Hau Wang

Abstract

A Gram-positive bacterium with antagonistic activity was isolated from the soil. It has been identified as Bacillus amyloliquefaciens strain V656 on the basis of 16S ribosomal DNA analysis and standard bacteriological tests. B. amyloliquefaciens V656 produced antifungal enzymes when it was grown in a medium containing shrimp and crab shell powder (SCSP) of marine waste. The antifungal enzymes displayed chitinase activities. Two extracellular antifungal chitinases (FI and FII) were purified and characterized, and their molecular weights, isoelectric points, pH and thermal stabilities, and antifungal activities were determined. The characteristics of V656 chitinases are similar to those of the known bacterial chitinases in terms of their isoelectric points, thermal instabilities, and lack of lysozyme activity. In contrast to other known bacterial chitinases, the unique characteristics of V656 chitinases include extremely low molecular weights and nearly neutral optimum pH. Furthermore, this is the first report of the isolation of chitinases from B. amyloliquefaciens that are active against fungi.

References

Aug 15, 1992·Experientia·J FlachP Jollès
Apr 15, 1988·European Journal of Biochemistry·B GrindeP Jollès
Mar 30, 1987·Biochemical and Biophysical Research Communications·D RobyA Toppan
Nov 1, 1995·Bioscience, Biotechnology, and Biochemistry·M UedaM Arai
Nov 14, 1997·Letters in Applied Microbiology·S PlebanI Chet
Apr 20, 2000·Enzyme and Microbial Technology·R S PatilM V Deshpande
May 1, 1995·Applied and Environmental Microbiology·L CherninI Chet
Oct 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M LegrandB Fritig
Jul 1, 1993·World Journal of Microbiology & Biotechnology·S A Shaikh, M V Deshpande

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Citations

Aug 18, 2012·Journal of Agricultural and Food Chemistry·Yao-Haur KuoSan-Lang Wang
Apr 19, 2012·Preparative Biochemistry & Biotechnology·Héla MezghanniNabil Zouari
Aug 8, 2008·Bioresource Technology·San-Lang WangYi-Hsuan Kuo
Sep 4, 2007·Bioresource Technology·San-Lang WangYue-Horng Yen
Mar 30, 2007·Toxicon : Official Journal of the International Society on Toxinology·Raimo MikkolaMirja S Salkinoja-Salonen
Sep 1, 2007·Molecular Plant Pathology·Ricardo B FerreiraArtur R Teixeira
Aug 8, 2013·Developmental and Comparative Immunology·Suchonma SookruksawongAnchalee Tassanakajon
Jul 19, 2013·Critical Reviews in Biotechnology·Anand NagpureRajinder K Gupta
Jun 16, 2012·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Tang-Bing CuiLi-Xiang Jiang
Sep 11, 2008·Current Microbiology·Maria Swiontek BrzezinskaMaciej Walczak
Mar 17, 2007·Critical Reviews in Biotechnology·Debaditya BhattacharyaRajinder K Gupta
Jun 25, 2010·Critical Reviews in Biotechnology·Chilukoti NeerajaAppa Rao Podile
Apr 17, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Luyi JiangChu Chu
Jan 1, 2013·3 Biotech·Faouzi Ben Rebah, Nabil Miled
Jul 25, 2019·World Journal of Microbiology & Biotechnology·Flávio Augusto CardozoAdalberto Pessoa
Jun 18, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·San-Lang WangAnh Dzung Nguyen
Jun 18, 2021·Journal of Applied Microbiology·Verónica RamírezAntonino Baez

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