PMID: 9545563Apr 18, 1998Paper

Purification and characterization of two insecticyanin-type proteins from the larval hemolymph of the Eri-silkworm, Samia cynthia ricini

Biochimica Et Biophysica Acta
H Saito

Abstract

Two different biliverdin-binding proteins, designated BBP-I and BBP-II, were purified from the larval hemolymph of the Eri-silkworm, Samia cynthia ricini. These proteins were readily isolated from the hemolymph of fifth instar larvae using two chromatographic steps, hydrophobic interaction chromatography and ion exchange chromatography. Both BBPs were easily separated by Q-Sepharose HP column chromatography. BBP-I has an apparent molecular weight of 24 kDa, as determined by gel-filtration and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Native BBP-II had a molecular weight of 48 kDa estimated by gel-filtration. SDS-PAGE revealed a single band with a molecular weight of 26 kDa. Moreover, the molecular weights of BBP-I and BBP-II were determined to be 20,468 and 22,708 by MALDI-TOF/MS (matrix-assisted laser desorption ionization-time of flight/mass spectrometry), respectively. On this basis, BBP-I and BBP-II molecules are assumed to be a monomer and a dimer, respectively. The blue color of BBPs collected from the hemolymph is attributed to the presence of biliverdin IX gamma, which is non-covalently and stoichiometrically bound to the apoprotein, based on absorbance maxima at 359 and 695 nm in methanol:HC...Continue Reading

References

Jan 1, 1990·Archives of Insect Biochemistry and Physiology·L M RiddifordB A Webb
Jan 29, 1986·Biochemical and Biophysical Research Communications·N H Haunerland, W S Bowers

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Citations

Oct 27, 1998·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·H SaitoY Kato
Mar 18, 2008·Toxicon : Official Journal of the International Society on Toxinology·Maria Esther Ricci-SilvaAna Marisa Chudzinski-Tavassi
Feb 27, 2007·Toxicon : Official Journal of the International Society on Toxinology·Linda Christian Carrijo-Carvalho, Ana Marisa Chudzinski-Tavassi
May 18, 2010·FEBS Letters·Ana Marisa Chudzinski-TavassiCleyson V Reis
Mar 23, 2017·Bulletin of Entomological Research·Hilal TuncaElisabeth Tabone
Mar 23, 2006·Archives of Insect Biochemistry and Physiology·Chi Won ChoiChi-Young Yun
Aug 17, 2005·Insect Biochemistry and Molecular Biology·Hong Ja KimSook Jae Seo

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