Purification and Characterization of Hydroxyquinol 1,2-Dioxygenase from Azotobacter sp. Strain GP1.

Applied and Environmental Microbiology
M LatusF Lingens

Abstract

Hydroxyquinol 1,2-dioxygenase was purified from cells of the soil bacterium Azotobacter sp. strain GP1 grown with 2,4,6-trichlorophenol as the sole source of carbon. The presumable function of this dioxygenase enzyme in the degradative pathway of 2,4,6-trichlorophenol is discussed. The enzyme was highly specific for 6-chlorohydroxyquinol (6-chloro-1,2,4-trihydroxybenzene) and hydroxyquinol (1,2,4-trihydroxybenzene) and was found to perform ortho cleavage of the hydroxyquinol compounds, yielding chloromaleylacetate and maleylacetate, respectively. With the conversion of 1 mol of 6-chlorohydroxyquinol, the consumption of 1 mol of O(inf2) and the formation of 1 mol of chloromaleylacetate were observed. Catechol was not accepted as a substrate. The enzyme has to be induced, and no activity was found in cells grown on succinate. The molecular weight of native hydroxyquinol 1,2-dioxygenase was estimated to 58,000, with a sedimentation coefficient of 4.32. The subunit molecular weight of 34,250 indicates a dimeric structure of the dioxygenase enzyme. The addition of Fe(sup2+) ions significantly activated enzyme activity, and metal-chelating agents inhibited it. Electron paramagnetic resonance data are consistent with high-spin iron(II...Continue Reading

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Citations

Feb 27, 2007·Applied and Environmental Microbiology·M A Sánchez, B González
Aug 25, 2015·Water, Air, and Soil Pollution·Dorota DomaradzkaDanuta Wojcieszyńska
Nov 9, 2006·Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes·Vasili M TravkinLudmila A Golovleva
Jun 10, 2004·Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes·Inna P Solyanikova, Ludmila A Golovleva
Oct 20, 2009·Applied and Environmental Microbiology·Maria Isabel M FerreiraToshiaki Kudo
Sep 22, 2019·Applied and Environmental Microbiology·Patrick Semana, Justin Powlowski
Jul 1, 2018·Antonie van Leeuwenhoek·Muralidharan MuthuGareth Lloyd-Jones
May 27, 2008·Journal of Bacteriology·Mariëlle J H MoonenWillem J H van Berkel

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