Purification and characterization of extracellular pectate lyase from Bacillus subtilis

Biochimie
W NasserJ Robert-Baudouy

Abstract

Bacillus subtilis strain SO113 secretes a pectate lyase which is produced during the exponential death phase of growth, just before sporulation. This extracellular pectate lyase, which produces unsaturated products from polygalacturonate, was purified 35-fold from the culture supernatant of Bacillus subtilis by a CM Sephadex chromatography. It has an isoelectric point of about 9.6 and an Mr of 42,000. Optimum activity occurred at pH 8.4 and at 42 degrees C. Calcium has a stimulative effect on the enzyme activity while EDTA leads to enzyme inactivation. The pectate lyase has a specific activity of 131 mumol of aldehyde groups per min and per mg of protein. The Km of the purified enzyme for polygalacturonic acid was 0.862 g.l-1 and the Vmax for polygalacturonic acid hydrolysis was 1.475 mumol of unsaturated products per min and per mg of protein. By using monoclonal antibodies raised against Erwinia chrysanthemi 3937 pectate lyases, it was shown that pectate lyases b and c of this strain are immunologically closely related to the Bacillus subtilis pectate lyase.

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jan 1, 1976·Analytical Biochemistry·A Bensadoun, D Weinstein
Feb 1, 1968·Archives of Biochemistry and Biophysics·F MoranM P Starr
Oct 15, 1958·Proceedings of the National Academy of Sciences of the United States of America·J Spizizen

❮ Previous
Next ❯

Citations

Sep 1, 1995·World Journal of Microbiology & Biotechnology·N E Karam, A Belarbi
Feb 18, 2005·Current Microbiology·Margarita SorianoF I Javier Pastor
Jun 2, 2005·Biotechnology Letters·Sathyanarayana N Gummadi, D Sunil Kumar
Dec 20, 1992·Journal of Molecular Biology·J JenkinsJ Robert-Baudouy
Oct 1, 1994·Nature Structural Biology·R PickersgillJ Robert-Baudouy
May 20, 2000·Biotechnology & Genetic Engineering Reviews·R A PradeA J Mort
Jul 31, 1999·Bioscience, Biotechnology, and Biochemistry·Y HatadaS Ito
Feb 10, 2009·Bioscience, Biotechnology, and Biochemistry·Wasana SukhumsiirchartTatsuji Sakamoto
Jan 16, 2009·Plant Physiology and Biochemistry : PPB·Wilaiwan ChotigeatAmornrat Phongdara
Sep 12, 2014·Extremophiles : Life Under Extreme Conditions·Masato SasakiKoki Horikoshi
Sep 20, 2005·Journal of Biotechnology·Barbara G Klug-SantnerGeorg M Guebitz
Jul 29, 1998·The Plant Journal : for Cell and Molecular Biology·C DomingoM C McCann
Apr 10, 1999·Journal of Bacteriology·M A BekriA Vande Broek

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biosynthetic Transformations

Biosyntheic transformtions are multi-step, enzyme-catalyzed processes where substrates are converted into more complex products in living organisms. Simple compounds are modified, converted into other compounds, or joined together to form macromolecules. Discover the latest research on biosynthetic transformations here.