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Purification and characterization of phosphodiesterase from Crotalus venom

Hoppe-Seyler's Zeitschrift für physiologische Chemie

Jul 1, 1975

Gabriele Philipps

PMID: 295

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Abstract

A procedure for the purification of phosphodiesterase from Crotalus venom on DEAE-cellulose at alkaline pH is described. The enzyme gives a single band in polyacrylamide gels and is free of contaminating nucleolytic enzymes. The molecular weight is about 115000. Concentration in an Amic...read more

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Polyacrylamide Gels
Chromatography, DEAE-Cellulose
Serum
Base
Denaturation
Endonuclease
bis(4-nitrophenyl)phosphate
Metazoa
DEAE-Cellulose
Transfer RNA
25
65
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  • Purification and characterization of phosphodiesterase from Crotalus venom

    Hoppe-Seyler's Zeitschrift für physiologische Chemie

    Jul 1, 1975

    Gabriele Philipps

    PMID: 295

    DOI:

    Abstract

    A procedure for the purification of phosphodiesterase from Crotalus venom on DEAE-cellulose at alkaline pH is described. The enzyme gives a single band in polyacrylamide gels and is free of contaminating nucleolytic enzymes. The molecular weight is about 115000. Concentration in an Amic...read more

    Mentioned in this Paper

    Polyacrylamide Gels
    Chromatography, DEAE-Cellulose
    Serum
    Base
    Denaturation
    25
    65

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