PMID: 6293580Nov 9, 1982

Purification and characterization of phosphoglycerate mutase isozymes from pig heart

Biochimica Et Biophysica Acta
R Bartrons, J Carreras

Abstract

The three isozymes of phosphoglycerate mutase from pig heart have been purified to homogeneity. The isozymes have a molecular weight of 57000 as determined by gel-filtration chromatography. Discontinuous gel electrophoresis in the presence of sodium dodecyl sulfate yields a single band with a molecular weight of 29000, indicating that the isozymes are dimers composed of subunits of similar mass. Hybridization experiments show that the three isozymes result from homodimeric and heterodimeric combinations of two different subunits. The two types of subunit differ in their heat lability and in the presence of -SH groups essential for enzymatic activity. No remarkable differences exist in the kinetic constants of the purified isozymes. The kinetic pattern is consistent with a 'ping-pong' mechanism. The homogeneous preparations of the three isozymes show intrinsic glycerate-2,3-P2 synthase activity and glycerate-2,3-P2 phosphatase activity which can be stimulated by glycolate-2-P.

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Citations

Dec 1, 1987·Developmental Biology·R FundeleE M Jägerbauer
Jan 1, 1993·Progress in Biophysics and Molecular Biology·L A Fothergill-Gilmore, P A Michels
Jan 1, 1983·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·A TaulerJ Carreras
Jan 1, 1985·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·R Fundele, W K Krietsch
Jan 1, 1986·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·G Pons, J Carreras
Jan 1, 1987·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·F Berrocal, J Carreras
Jan 1, 1988·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·M O PrehuR Rosa
Jun 1, 1996·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·N Durany, J Carreras
Jul 31, 2002·Molecular Pathology : MP·N DuranyJ Carreras
Sep 28, 2001·Biochemical and Biophysical Research Communications·T Hayashi, Y Matuo
Jan 1, 1995·Muscle & Nerve. Supplement·S TsujinoS DiMauro
Oct 12, 2007·Biotechnology and Bioengineering·Joon Chong YeeWei-Shou Hu
Dec 29, 1989·Biochemical and Biophysical Research Communications·J Castellà-EscolàF Climent
Jun 28, 1985·Biochemical and Biophysical Research Communications·G PonsJ Carreras
Jun 14, 2005·Biochimica Et Biophysica Acta·Pedro de AtauriJosé Carreras
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Dec 25, 2007·Biochemical and Biophysical Research Communications·Feng LuNing Guo

Related Concepts

Metazoa
Chromatography
Drug Stability
Isoelectric Point
Alloenzymes
Myocardium
Phosphoglycerate Mutase
Phosphotransferases
Family suidae
Macromolecular Compounds

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