PMID: 9183020May 1, 1997Paper

Purification and characterization of flavoproteins and cytochromes from the yellow bioluminescence marine bacterium Vibrio fischeri strain Y1

European Journal of Biochemistry
V N Petushkov, J Lee

Abstract

Several flavoproteins and cytochromes that occur as major components in extracts of the yellow bioluminescence Y1 strain of the marine bacterium Vibrio fischeri have been purified and characterized with respect to their mass (SDS/PAGE and matrix-assisted laser-desorption/ionization MS), chromatographic properties, N-terminal sequence, and spectroscopy (absorption, fluorescence emission and anisotropy decay). The investigated proteins were as follows: yellow fluorescence protein (YFP) with bound riboflavin, FMN or 6,7-dimethyl-8-ribityllumazine; a blue fluorescence protein (BFP) with bound 6,7-dimethyl-8-ribityllumazine, riboflavin, or 6-methyl-7-oxo-8-ribityllumazine; thioredoxin reductase with FAD as ligand; and two c-type diheme cytochromes, c551 and c554. We present evidence that the riboflavin-bound YFP has an N-terminal sequence corresponding to that published for the dimeric YFP. We show that an equilibrium replacement of the riboflavin can be made with excess lumazine derivative and that lumazine-bound YFP has different bioluminescence properties to those of the lumazine protein from Photobacterium leiognathi. BFP is a different protein again, and in the bacterial lysate it occurs in multiple forms, ligated to either rib...Continue Reading

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Citations

Jan 11, 2012·Protein & Cell·Maxim S TitushinZhi-Jie Liu
Jul 13, 2005·Natural Product Reports·Markus Fischer, Adelbert Bacher
Aug 8, 2001·European Journal of Biochemistry·S EberhardtA Bacher
Jun 8, 2011·Microbiology and Molecular Biology Reviews : MMBR·Charles A Abbas, Andriy A Sibirny
Jul 8, 2008·Journal of Molecular Biology·Lorenz ChatwellMarkus Fischer
Nov 30, 2018·Photochemistry and Photobiology·John LeeAntonie J W G Visser
Jun 10, 2005·The Journal of Biological Chemistry·Boris IllarionovMarkus Fischer
Sep 1, 2010·EcoSal Plus·Markus Fischer, Adelbert Bacher
Feb 27, 2008·Archives of Biochemistry and Biophysics·Markus Fischer, Adelbert Bacher

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