Purification and chemical composition of reticulate bodies of the meningopneumonitis organisms.

Journal of Bacteriology
A TamuraN Higashi


Reticulate bodies of the meningopneumonitis (MP) microorganism were purified from L cells 18 hr after infection by the combination of differential centrifugation in 30% sucrose solution and potassium tartrate density gradient centrifugation. It was ascertained by electron microscopy that purified preparations of reticulate bodies obtained were almost entirely free of host-cell components and of infectious elementary bodies of MP microorganisms. Purified reticulate bodies were easily disrupted by mechanical agitation, and it was observed in shadowed preparation that ribosome-like particles 15 mmu in diameter were scattered from broken reticulate bodies. In shadowed preparations, reticulate bodies were found to range in size from 1.0 to 1.6 mu in diameter, but in cross-section the range was 0.5 to 1.0 mu. In these preparations, the purified reticulate bodies were irregular in shape, round or oval, and were composed of rather homogenous, amorphous, or reticulate material with moderate density. Some particles exhibited a less-dense internal structure, in which a coarse fibrous reticulum was seen. Chemical fractionation of (32)P-labeled purified reticulate bodies showed that they contained three times more ribonucleic acid (RNA) tha...Continue Reading


Feb 1, 1954·The Journal of Experimental Medicine·R DULBECCO, M VOGT
Feb 1, 1961·The Journal of Biophysical and Biochemical Cytology·J H LUFT
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Mar 1, 1964·Virology·R A ERLANDSON, E G ALLEN
Jan 1, 1965·Journal of Molecular Biology·A TAMURA, M IWANAGA

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