Abstract
NADPH-cytochrome P-450 reductase has been purified to apparent homogeneity from liver microsomes of beta-naphthoflavone-treated rats and rainbow trout. The apparent monomeric molecular weights were 75,000 and 77,000 for the rat and trout, respectively. Differences in amino acid composition were observed, particularly for lysine, glycine, threonine, and tyrosine. Analysis of the flavin composition showed that there were 0.97 mol of FAD and 0.92 mol of FMN per mol of rat reductase, whereas the values for the trout enzyme were 1.06 and 0.76 for FAD and FMN, respectively. Trout NADPH-cytochrome c reductase was inhibited by anti-rat antibody, but not to the same extent as was the rat enzyme. No precipitin lines between the trout reductase and rat antibody were observed on Ouchterlony plates. Peptide patterns, on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, following limited proteolysis were also markedly different. The trout enzyme was as effective, catalytically, as the rat enzyme in a reconstituted system that contained purified rat cytochrome P-448 and lipid. Comparison of ethoxyresorufin-O-deethylase temperature profiles with various combinations of purified trout and rat P-448, reductase, and lipid, in membranous ...Continue Reading
References
Mar 16, 1976·European Journal of Biochemistry·P Pajot
Jan 1, 1977·Comparative Biochemistry and Physiology. C: Comparative Pharmacology·R F AddisonD E Willis
Jan 19, 1979·Biochimica Et Biophysica Acta·H TaniguchiR Sato
Sep 28, 1979·Molecular and Cellular Biochemistry·D W Nebert
Jun 1, 1975·FEBS Letters·C S Yang
Oct 1, 1978·Environmental Research·E H Gerhart, R M Carlson
Jan 1, 1979·Pharmacology & Therapeutics·F P Guengerich
Apr 1, 1979·Bulletin of Environmental Contamination and Toxicology·B KurelecR K Zahn
Jul 1, 1979·Toxicology and Applied Pharmacology·C R Elcombe, J J Lech
Jun 14, 1978·Biochemical and Biophysical Research Communications·F P Guengerich
May 1, 1976·Life Sciences·G T Miwa, A K Cho
May 1, 1976·Acta Pharmacologica Et Toxicologica·J T AhokasN T Kärki
Aug 1, 1976·Journal of Biochemistry·Y Imai
May 1, 1973·Analytical Biochemistry·E J Faeder, L M Siegel
Sep 14, 1968·Nature·H MetzgerJ E Vinton
Apr 1, 1968·Comparative Biochemistry and Physiology·D R Buhler, M E Rasmusson
Mar 1, 1971·Archives of Biochemistry and Biophysics·M R Franklin, R W Estabrook
Aug 15, 1970·Nature·U K Laemmli
Jan 1, 1981·Comparative Biochemistry and Physiology. C: Comparative Pharmacology·M J MelanconJ J Lech
Jan 1, 1981·Comparative Biochemistry and Physiology. C: Comparative Pharmacology·L Förlin, U Lidman
May 1, 1981·Journal of Toxicology and Environmental Health·R B FranklinJ J Lech
Feb 15, 1980·Biochemical Pharmacology·T HanssonJ A Gustafsson
Jul 1, 1980·Toxicology and Applied Pharmacology·L Förlin
Jan 1, 1980·Pharmacology & Therapeutics·B S Masters, R T Okita
Dec 1, 1980·Archives of Biochemistry and Biophysics·F P Guengerich, M V Martin
Mar 31, 1981·Biochemistry·T IyanagiF K Anan
Apr 28, 1981·Biochemistry·F P GuengerichM B Mitchell
Apr 28, 1981·Biochemistry·F P GuengerichP S Mason
Nov 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·L M Hjelmeland
Aug 27, 1982·Biochimica Et Biophysica Acta·D E Williams, D R Buhler
Jun 30, 1981·Toxicology and Applied Pharmacology·M J VodicnikJ J Lech
Dec 1, 1981·Archives of Biochemistry and Biophysics·A I ArchakovK N Myasoedova
May 1, 1980·Analytical Biochemistry·H W Chang, E Bock
Jun 15, 1980·Toxicology and Applied Pharmacology·M O James, J R Bend
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER
Citations
Apr 1, 1994·Journal of Biochemical Toxicology·P Lemaire, D R Livingstone
Dec 7, 2011·Ecotoxicology·B LopesM J Bebianno
Feb 9, 1990·Biochimica Et Biophysica Acta·C L MirandaD R Buhler
Jan 5, 1995·Biochimica Et Biophysica Acta·H ImaishiH Ohkawa
Feb 1, 1995·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·N TakahashiD E Williams
Oct 1, 1995·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·N TakahashiG S Bailey
Jan 1, 1985·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·M BizriR Got
Jan 1, 1985·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·H FukushimaY Nozawa
Jan 1, 1989·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·T Andersson, E Nilsson
Jan 1, 1985·Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology·G T AnkleyR A White
Jan 1, 1985·Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology·G M Dalich, R E Larson
Jan 1, 1987·Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology·G MonodJ L Rivière
Jan 1, 1989·Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology·D Schlenk, D R Buhler
Mar 1, 1995·Comparative Biochemistry and Physiology. Part C, Pharmacology, Toxicology & Endocrinology·N TakahashiG S Bailey
Oct 1, 1985·Chemico-biological Interactions·R L BinderJ J Lech
Jun 1, 1997·Comparative Biochemistry and Physiology. Part C, Pharmacology, Toxicology & Endocrinology·P Lemaire, D R Livingstone
Feb 11, 1999·Comparative Biochemistry and Physiology. Part C, Pharmacology, Toxicology & Endocrinology·D R Buhler, J L Wang-Buhler
Feb 1, 1989·Clinical and Experimental Pharmacology & Physiology·M E McManusD J Birkett
Apr 3, 2008·Marine Pollution Bulletin·A SerafimM J Bebianno
May 1, 1984·Toxicology and Applied Pharmacology·R L Binder, J J Stegeman
Nov 23, 2006·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Azra Bozcaarmutlu, Emel Arinç
Aug 29, 2006·Aquatic Toxicology·Diane L NabbXing Han
Sep 15, 2007·Environmental Pollution·Alexandra AbrahamsonEven H Jørgensen
Mar 15, 1985·Biochemical and Biophysical Research Communications·P Gurumurthy, G J Mannering
Jun 1, 1984·Archives of Biochemistry and Biophysics·D E WilliamsB S Masters
Dec 14, 2002·Journal of Biochemical and Molecular Toxicology·Emel ArinçHaydar Lelik
Nov 13, 2007·Journal of Biochemical and Molecular Toxicology·Azra Bozcaarmutlu
May 20, 1998·Archives of Biochemistry and Biophysics·Y H YangD R Buhler
Jun 26, 1987·Journal of Chromatography·J UrenjakL Lumper
May 31, 1991·Biochemical and Biophysical Research Communications·I BenvenisteF Durst
Apr 1, 1987·Analytical Biochemistry·C M ArdiesC S Lieber