PMID: 9543008May 23, 1998Paper

Purification and crystallization of complexes modeling the active state of the fragile histidine triad protein

Protein Engineering
C BrennerL D Barnes

Abstract

Fragile histidine triad protein (Fhit) is a diadenosine triphosphate (ApppA) hydrolase encoded at the human chromosome 3 fragile site which is frequently disrupted in tumors. Reintroduction of FHIT coding sequences to cancer cell lines with FHIT deletions suppressed the ability of these cell lines to form tumors in nude mice even when the reintroduced FHIT gene had been mutated to allow ApppA binding but not hydrolysis. Because this suggested that the tumor suppressor activity of Fhit protein depends on substrate-dependent signaling rather than ApppA catabolism, we prepared two crystalline forms of Fhit protein that are expected to model its biologically active, substrate-bound state. Wild-type and the His96Asn forms of Fhit were overexpressed in Escherichia coli, purified to homogeneity and crystallized in the presence and absence of ApppA and an ApppA analog. Single crystals obtained by vapor diffusion against ammonium sulfate diffracted X-rays to beyond 2.75 A resolution. High quality native synchrotron X-ray data were collected for an orthorhombic and a hexagonal crystal form.

References

Apr 28, 1968·Journal of Molecular Biology·B W Matthews
Apr 5, 1996·Cell·G SozziC M Croce
Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·L VirgilioC M Croce
Feb 12, 1998·Proceedings of the National Academy of Sciences of the United States of America·Z SiprashviliK Huebner

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Citations

Sep 25, 1999·Journal of Cellular Physiology·C BrennerK Huebner
Sep 29, 2000·Pharmacology & Therapeutics·A G McLennan
Feb 15, 2000·The Journal of Biological Chemistry·A DraganescuC Brenner
May 20, 1998·Proceedings of the National Academy of Sciences of the United States of America·H C PaceC Brenner
Mar 10, 2004·Proceedings of the National Academy of Sciences of the United States of America·Yuri PekarskyCarlo M Croce
Dec 25, 2009·Journal of Cancer Research and Clinical Oncology·Md Imtaiyaz HassanFaizan Ahmad
Aug 25, 2004·Biochemical and Biophysical Research Communications·Makito HiranoSatoshi Ueno
Dec 9, 2014·Cell Cycle·Jennifer A Boylston, Charles Brenner
Mar 21, 2006·Journal of Cellular Physiology·Manuela CampiglioLuca Roz
Aug 13, 2008·FEBS Letters·Andrzej GuranowskiWojciech J Stec
Nov 5, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Xiaohong QinYuequan Shen
Aug 14, 1999·The Journal of Biological Chemistry·A R ChaudhuriL D Barnes
Jan 15, 2014·Acta Crystallographica. Section F, Structural Biology Communications·Alexander McPherson, Jose A Gavira
Mar 29, 2000·Protein Expression and Purification·T PawelczykA Matecki
Jun 11, 2021·Scientific Reports·Xianglian ZhouXisong Ke

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