Purification and crystallization of Phd, the antitoxin of the phd/doc operon.

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Abel Garcia-PinoRemy Loris

Abstract

The antitoxin Phd from the phd/doc module of bacteriophage P1 was crystallized in two distinct crystal forms. Crystals of His-tagged Phd contain a C-terminally truncated version of the protein and diffract to 2.20 A resolution. Crystals of untagged Phd purified from the Phd-Doc complex diffract to 2.25 A resolution. These crystals are partially merohedrally twinned and contain the full-length version of the protein.

References

Aug 5, 1992·Journal of Molecular Biology·P Bernard, M Couturier
Feb 7, 2003·Proceedings of the National Academy of Sciences of the United States of America·Anton MeinhartWolfram Saenger
May 14, 2003·The Journal of Biological Chemistry·Remy LorisLode Wyns
Jun 6, 2003·Molecular Microbiology·Susanne K Christensen, Kenn Gerdes
Sep 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·UNKNOWN Collaborative Computational Project, Number 4
May 3, 2005·Nature Reviews. Microbiology·Kenn GerdesAnders Løbner-Olesen
Nov 1, 2005·Trends in Biochemical Sciences·Lieven ButsRemy Loris
Sep 30, 2006·Journal of Molecular Biology·Tobias MadlKlaus Zangger
Oct 6, 2006·Molecular Microbiology·Mikkel Christensen-Dalsgaard, Kenn Gerdes
Oct 31, 2006·PLoS Genetics·Hanna Engelberg-KulkaRonen Hazan
Jul 28, 2007·Protein Science : a Publication of the Protein Society·Monika ObererWalter Keller
Apr 10, 2008·Proceedings of the National Academy of Sciences of the United States of America·Mohan LiuNancy A Woychik
Sep 2, 2008·The Journal of Biological Chemistry·Abel Garcia-PinoRemy Loris
Nov 11, 2008·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Abel Garcia-PinoRemy Loris
Apr 14, 2009·Molecular Cell·Carolyn A WorbyJack E Dixon
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Paul D AdamsPeter H Zwart
Jan 1, 1997·Methods in Enzymology·Zbyszek Otwinowski, Wladek Minor

❮ Previous
Next ❯

Citations

Jul 4, 2018·Acta Crystallographica. Section F, Structural Biology Communications·Dukas JurėnasAbel Garcia-Pino

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.