Purification and crystallization of the yeast elongation factor eEF2

Acta Crystallographica. Section D, Biological Crystallography
Rene JørgensenGregers Rom Andersen

Abstract

Crystals of the Saccharomyces cerevisiae elongation factor 2 (eEF2) in complex with GDP were obtained with the vapour-diffusion technique after rapid purification from industrial yeast. The crystals diffract to 2.85 A and belong to the space group P2(1)2(1)2(1). A yeast strain expressing a functional histidine-tagged eEF2 as the only form of the protein further allows facilitated purification of the factor for both structural and functional studies.

Citations

Sep 27, 2007·BMC Evolutionary Biology·Tetyana Nosenko, Debashish Bhattacharya
Apr 9, 2016·Molecular Cell·Alexey PetrovJoseph D Puglisi
Nov 12, 2013·Journal of Molecular Biology·Erin L BolandGregory M K Poon
Jul 19, 2002·Yeast
Aug 19, 2005·Nature·René JørgensenGregers R Andersen
Apr 15, 2003·Nature Structural Biology·Rene JørgensenGregers Rom Andersen
Sep 5, 2006·The Journal of Biological Chemistry·Pedro A OrtizTerri Goss Kinzy
Nov 4, 2006·The Journal of Biological Chemistry·Rikke SøeGregers R Andersen
Aug 23, 2007·Clinical Chemistry·Christopher BachranHendrik Fuchs
Aug 19, 2004·The Journal of Biological Chemistry·René JørgensenGregers Rom Andersen
Jul 16, 2013·The Journal of Biological Chemistry·Maria K Mateyak, Terri Goss Kinzy
Feb 9, 2021·The EMBO Journal·Namit RanjanDaniel N Wilson
Jan 9, 2021·Proceedings of the National Academy of Sciences of the United States of America·Martin Y NgBarry S Cooperman
Dec 24, 2020·Human Molecular Genetics·Maria J Nabais SáBert B A de Vries
Feb 12, 2019·Protein Expression and Purification·Rasmus Kock FlygaardLasse Bohl Jenner

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