Purification and enrichment of specific chromatin loci.

Nature Methods
Mathilde GauchierJérôme Déjardin

Abstract

Understanding how chromatin is regulated is essential to fully grasp genome biology, and establishing the locus-specific protein composition is a major step toward this goal. Here we explain why the isolation and analysis of a specific chromatin segment are technically challenging, independently of the method. We then describe the published strategies and discuss their advantages and limitations. We conclude by discussing why significant technology developments are required to unambiguously describe the composition of small single loci.

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Citations

Jan 31, 2020·Nature Reviews. Molecular Cell Biology·Michiel Vermeulen, Jérôme Déjardin
Aug 28, 2020·Frontiers in Cell and Developmental Biology·William A Scott, Eric I Campos
Nov 11, 2020·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Isabel EspejoSergi Aranda
Oct 17, 2020·Molecular & Cellular Proteomics : MCP·Guido van Mierlo, Michiel Vermeulen
Feb 9, 2021·Molecular & Cellular Proteomics : MCP·Guido van Mierlo, Michiel Vermeulen
Apr 17, 2021·Genome Biology·Anil Panigrahi, Bert W O'Malley
Nov 22, 2020·Current Opinion in Genetics & Development·Sjoerd Jd Tjalsma, Wouter de Laat
Aug 4, 2021·Current Opinion in Plant Biology·Milad AlizadehLiang Song
Aug 24, 2021·Frontiers in Genetics·Marthe Behrends, Olivia Engmann

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Methods Mentioned

BETA
immunoprecipitation
ChIP
pulldown
PCR
affinity purification
enzymatic treatments
ion mobility spectrometry
ChIP-seq
transfections

Software Mentioned

CLASP
BioID
SILAC
Epi
- Decoder
HyCCAPP
CAPLOCUS
PICh
CasID
Decoder

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