Abstract
In this study, among the 10 genes that encode putative β-glucosidases in the glycoside hydrolase family 3 (GH3) with a signal peptide in the Aspergillus oryzae genome, we found a novel gene (AO090038000425) encoding β-1,6-glucosidase with a substrate specificity for gentiobiose. The transformant harboring AO090038000425, which we named bglH, was overexpressed under the control of the improved glaA gene promoter to form a small clear zone around the colony in a plate assay using 4-methylumbelliferyl β-d-glucopyranoside as the fluorogenic substrate for β-glucosidase. We purified BglH to homogeneity and enzymatically characterize this enzyme. The thermal and pH stabilities of BglH were higher than those of other previously studied A. oryzae β-glucosidases, and BglH was stable over a wide temperature range (4°C-60°C). BglH was inhibited by Hg(2+), Zn(2+), glucono-δ-lactone, glucose, dimethyl sulfoxide, and ethanol, but not by ethylenediaminetetraacetic acid. Interestingly, BglH preferentially hydrolyzed gentiobiose rather than other oligosaccharides and aryl β-glucosides, thereby demonstrating that this enzyme is a β-1,6-glucosidase. To the best of our knowledge, this is the first report of the purification and characterization of ...Continue Reading
References
Feb 19, 1975·Biochimica Et Biophysica Acta·K SanoT Harada
Aug 15, 1970·Nature·U K Laemmli
May 1, 1964·The Biochemical Journal·G A LevvyJ Conchie
Aug 1, 1995·Plant Physiology·A Falk, L Rask
Feb 1, 1995·Plant Physiology·D P DharmawardhanaJ E Carlson
Dec 1, 1996·Applied Microbiology and Biotechnology·N KitamotoN Tsukagoshi
Oct 6, 1998·Applied and Environmental Microbiology·C RiouP Barre
Feb 15, 2000·The Journal of Biological Chemistry·S DanO Shoseyov
May 2, 2001·Applied Microbiology and Biotechnology·C H DeckerP Schreier
Dec 19, 2002·Critical Reviews in Biotechnology·Yukti BhatiaV S Bisaria
Apr 23, 2005·Chembiochem : a European Journal of Chemical Biology·Susan M HancockBenjamin G Davis
Oct 20, 2005·Journal of Bioscience and Bioengineering·Kengo MatsumuraOsamu Akita
Dec 24, 2005·Nature·Masayuki MachidaHisashi Kikuchi
May 3, 2006·Biochimica Et Biophysica Acta·James LangstonFeng Xu
Aug 10, 2006·Applied Microbiology and Biotechnology·Takeshi TsukadaMasahiro Samejima
Sep 30, 2008·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Masayuki MachidaKatsuya Gomi
May 22, 2010·Cellular and Molecular Life Sciences : CMLS·James R Ketudat Cairns, Asim Esen
Sep 16, 2011·Journal of Agricultural and Food Chemistry·Yuxia WangYan Xu
Jan 5, 2013·Applied Biochemistry and Biotechnology·Haiyan HeZhiqun Liang
May 11, 2013·The Journal of Biological Chemistry·Taku UchiyamaKatsuro Yaoi
May 6, 2015·Journal of Bioscience and Bioengineering·Kanako KudoKatsuya Gomi
Citations
Sep 27, 2016·Bioresource Technology·Ramkumar B Nair, Mohammad J Taherzadeh
Mar 28, 2018·The Biochemical Journal·Hideyuki TakahashiTakumi Takeda
Nov 30, 2019·Applied Microbiology and Biotechnology·Tomohiko MatsuzawaKatsuro Yaoi
Apr 27, 2019·Scientific Reports·Jianfeng MeiGuoqing Ying
Jan 13, 2016·The Journal of Biological Chemistry·Tomohiko MatsuzawaKatsuro Yaoi
Nov 21, 2019·Mikrochimica Acta·Ziping LiuShasha Liu
Apr 26, 2020·Scientific Reports·Lummy Maria Oliveira MonteiroMaria de Lourdes Teixeira de Moraes Polizeli
Jan 1, 2016·3 Biotech·Peizhou YangShaotong Jiang
Jan 29, 2021·Journal of Food Science and Technology·Min SuChunhong Piao