Purification and identification of a 28-kDa calcium-regulated heat-stable protein. A novel secretagogue-regulated phosphoprotein in exocrine pancreas.

The Journal of Biological Chemistry
Guy E GroblewskiJ A Williams

Abstract

This study reports the purification and identification of a novel 28 kDa phosphoprotein from rat pancreatic acini, previously described as being highly regulated by calcium mobilizing secretagogues, which we have designated calcium-regulated heat-stable protein 28 (CRHSP-28). Internal amino acid sequences of purified CRHSP-28 were obtained following trypsin digestion and found to match with >95% identity the predicted amino acid sequence of a novel cDNA recently identified as being highly expressed in human breast carcinomas. Verification that this cDNA codes for human CRHSP-28 was demonstrated by the ability of antiserum raised against purified rat CRHSP-28 to recognize the recombinant human protein when expressed in bacteria. Furthermore, this antibody was found to specifically react with CRHSP-28 in rat acini following one- and two-dimensional electrophoresis and underwent a marked acidic shift in mobility after cholecystokinin stimulation, a phenomenon indicative of an increase in its phosphorylation. CRHSP-28 is predicted to be extremely hydrophilic, is phosphorylated entirely on serine residues, and bears little homology to any known proteins. Finally, the distribution of the CRHSP-28 protein in various rat tissues reveal...Continue Reading

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Citations

Sep 11, 2010·The Journal of Biological Chemistry·Michelle A FalkowskiGuy E Groblewski
Jun 4, 2011·American Journal of Physiology. Gastrointestinal and Liver Physiology·Michelle A FalkowskiGuy E Groblewski
Oct 4, 2008·American Journal of Physiology. Gastrointestinal and Liver Physiology·Catherine S ChewHan Zhang
Mar 14, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·Ning WengGuy E Groblewski
Oct 12, 2001·Biochemical and Biophysical Research Communications·P SathasivamJ A Byrne
May 7, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Jennifer A ByrneRobert K Bright
Nov 24, 2004·Biochemical and Biophysical Research Communications·Rose BoutrosJennifer A Byrne
May 30, 2009·Biochemical and Biophysical Research Communications·SaeHong LeeJohn A Williams
Feb 24, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·Diana D H ThomasGuy E Groblewski
Jul 10, 2002·The Journal of Biological Chemistry·Diana D H ThomasGuy E Groblewski
Sep 12, 2018·Journal of Cellular Biochemistry·Mihyang HaYun Hak Kim
Jul 23, 2013·American Journal of Physiology. Gastrointestinal and Liver Physiology·Scott W MessengerGuy E Groblewski
Dec 25, 2009·American Journal of Physiology. Cell Physiology·Diana D H ThomasGuy E Groblewski
Jun 1, 2001·The Journal of Biological Chemistry·D D ThomasG E Groblewski
Aug 26, 1998·The Journal of Biological Chemistry·G E GroblewskiJ A Williams
Jun 13, 2003·American Journal of Physiology. Gastrointestinal and Liver Physiology·Claus SchäferGuy E Groblewski
Aug 2, 2003·American Journal of Physiology. Gastrointestinal and Liver Physiology·Kala M KasparGuy E Groblewski
Nov 24, 1999·Molecular and Cellular Biology·S E RossO A MacDougald
Jan 14, 1999·The American Journal of Physiology·G E GroblewskiS A Ernst

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