PMID: 8461423Feb 1, 1993Paper

Purification and identification of a serum protein increased by anthelmintic drugs for Dirofilaria immitis in dogs

The Journal of Veterinary Medical Science
N TosaM Saito

Abstract

Polyacrylamide gel electrophoretic analysis of canine serum protein has revealed that the administration of anthelmintics elicits an increase in a certain serum protein. This protein, named PT60, was partially purified by ammonium sulfate fractionation and preparative electrophoresis. The purified PT60 gave a single band with the molecular size of 53 kDa in sodium dodecyl sulfate-polyacrylamide gel electrophoresis under non-reducing conditions. After reduction with 2-mercaptoethanol, two bands appeared at 35 kDa and 17 kDa, indicating that PT60 consists of two subunits which are linked with each other by disulfide bonds. PT60 had the capacity to bind to hemoglobin. In an immunodiffusion test, an antiserum against PT60 cross-reacted with canine haptoglobin (Hp). N-terminal amino acid sequences of two PT60 subunits were identical to those of alpha and beta subunits of canine Hp, respectively. Thus, PT60 was identified as Hp.

Citations

Apr 1, 1995·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·K MominokiM Saito
Jul 21, 2004·The Veterinary Record·S Martínez-SubielaJ J Cerón
Aug 21, 2003·American Journal of Veterinary Research·Silvia Martínez-SubielaJosé J Cerón
May 8, 2008·The Journal of Veterinary Medical Science·Sunny C H YuehSimon J T Mao
Oct 8, 1999·Journal of Chromatography. B, Biomedical Sciences and Applications·S J Yang, S J Mao
May 28, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Chun Yi LiauSimon J T Mao
May 26, 2004·The Veterinary Journal·H MurataM Yoshioka

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Anthelmintics (ASM)

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.

Anthelmintics

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.

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