Purification and identification of an IgE suppressor from strawberry in an in vitro immunization system.

Cytotechnology
Akira IwamotoHiroharu Kawahara

Abstract

We purified and identified an IgE suppressor from the strawberry 'Toyonoka', based on the decrease of IgE production in in vitro immunization (IVI). Gel filtration experiment indicated that fractions in a 15-48 kDa range and <10 kDa have an IgE suppressive activity. Furthermore, the fraction in 15-48 kDa was subjected to chromatofocusing and found to have activities at isoelectric points, pI 6.0, 7.0, and 8.0-9.2. We focused on the active fractions of pI 8.0-9.2 and the purified a large amount of strawberry extracts by cation exchange resins in batch. A purified 39 kDa protein showed homology to plant glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in N-terminal amino acid sequence and had GAPDH enzymatic activity. Nucleotide sequence and deduced amino acid sequence of the obtained cDNA clone of the protein matched with the sequence of Fragaria x ananassa GAPDH in the GenBank with >98% identical nucleotides and >99% identical amino acids, respectively. The purified strawberry GAPDH suppressed total IgE production in IVI in a dose-dependent manner. From these results, we identified GAPDH as IgE suppressor in the strawberry. Our study may be applicable to the development of new methods to relieve allergic conditions using GAPDH ...Continue Reading

References

Jan 1, 1986·Annual Review of Immunology·H MetzgerR Quarto
Dec 10, 1999·Nature·S T Holgate
Dec 10, 1999·Nature·D B Corry, F Kheradmand
Aug 30, 2005·Biochimica Et Biophysica Acta·Ryoichi YamajiYoshihisa Nakano
Feb 16, 2006·Current Allergy and Asthma Reports·Masato Kubo, Hiromasa Inoue
Apr 1, 2006·Biochimica Et Biophysica Acta·Makoto R HaraAkira Sawa
May 15, 2007·European Journal of Nutrition·Satomi YanoHirofumi Tachibana
Jun 7, 2007·Journal of Agricultural and Food Chemistry·Kjersti AabyGrete Skrede
Jan 24, 2008·Journal of Agricultural and Food Chemistry·Yanjun ZhangDavid Heber
Apr 28, 2010·Annals of Clinical and Laboratory Science·Ehab HassanainTamar A Smith-Norowitz

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Citations

Jun 20, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Akinobu TanakaKuniyoshi Shimizu
Feb 17, 2019·Cytotechnology·Akira IwamotoHiroharu Kawahara
Dec 12, 2020·Journal of Agricultural and Food Chemistry·Akira IwamotoTeruyoshi Yanagita

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