Purification and identification of antibacterial phenolics from Tripodanthus acutifolius leaves

Journal of Applied Microbiology
José R SoberónMarta A Vattuone

Abstract

To perform an activity-guided purification, identification and quantification of antibacterial compounds from Tripodanthus acutifolius infusion. To validate the antibacterial activity of purified substances. Bioautographic methods were employed as screening assays for purifying bioactive substances. Purification procedures included sephadex LH-20 column chromatography and reverse phase HPLC. Identification was achieved by spectroscopic methods (UV-Vis, MS, NMR and polarimetry) and chromatographic assays (paper chromatography and HPLC). Antibacterial activity was studied by microdilution, colony count and photometric assays, Sytox green stain and transmission electron microscopy (TEM). Four glycoflavonoids (rutin, nicotiflorin, hyperoside and isoquercitrin) and an unusual phenylbutanoid glycoside (tripodantoside) were purified and identified. Tripodantoside was found at 6.59 +/- 0.82 g per 100 g of dry leaves. The flavonoids showed bactericidal effect at a concentration of 4 mg ml(-1) against Staphylococcus aureus and Pseudomonas aeruginosa strains from American Type Culture Collection, while tripodantoside was almost four times more active than those compounds, with a minimum bactericidal concentration = 1.024 mg ml(-1) against...Continue Reading

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Citations

Jun 5, 2014·Journal of Bioscience and Bioengineering·José R SoberónMarta A Vattuone
Dec 17, 2014·Frontiers in Plant Science·Aleksandra SkiryczJose O Siqueira
May 18, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Verena Spiegler

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