PMID: 6986175Jan 24, 1980Paper

Purification and immunohistochemical localization of rat liver glutathione peroxidase

Biochimica Et Biophysica Acta
S YoshimuraK Watanabe

Abstract

Glutathione peroxidase was purified from the rat liver to give a single protein band in polyacrylamide gel electrophoresis. Rabbits were immunized with this purified enzyme, and a highly specific anti-glutathione peroxidase antiserum was obtained. Using this antibody, an immunohistochemical technique (the indirect method of peroxidase-labeled antibody) was applied to study the localization of the enzyme in the liver cells. On immunohistochemical observation, glutathione peroxidase was localized exclusively in the cytoplasm of hepatocytes, and a stronger 'immuno-staining' was exhibited in the peripheries of the hepatic lobules than in the central zone.

References

Jun 30, 1975·Annals of the New York Academy of Sciences·P K Nakane
Aug 23, 1976·Biochemical and Biophysical Research Communications·R A Lawrence, R F Burk
May 23, 1976·Biochemical and Biophysical Research Communications·J R Prohaska, H E Ganther
Dec 1, 1974·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·I W McLean, P K Nakane
Dec 1, 1974·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·P K Nakane, A Kawaoi
Sep 1, 1974·The American Journal of Clinical Nutrition·A L Tappel
Feb 9, 1973·Science·J T RotruckW G Hoekstra
Sep 10, 1969·Biochemical and Biophysical Research Communications·V MasseyP A Sullivan
Apr 19, 1968·Biochemical and Biophysical Research Communications·C Little, P J O'Brien
Apr 1, 1966·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·R C Graham, M J Karnovsky

❮ Previous
Next ❯

Citations

Jan 13, 2006·Pflügers Archiv : European journal of physiology·Shuichi UchiyamaTetsuro Tamaki
Feb 1, 1992·Mechanisms of Ageing and Development·J SastreJ Viña
Feb 7, 2001·Journal of the National Cancer Institute·J HamadaT Moriuchi
May 8, 2001·Journal of Gastroenterology and Hepatology·Y H ChungD J Suh
May 20, 2017·Acta Histochemica Et Cytochemica·Masanori Murakoshi, Robert Yoshiyuki Osamura
Oct 1, 1986·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·B Ketterer
May 29, 2014·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·T DepremG Nur
Dec 1, 1994·Asia-Oceania Journal of Obstetrics and Gynaecology·G SaitoT Moriuchi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.