PMID: 6170346Sep 18, 1981Paper

Purification and immunological studies of glutathione reductase from rat liver. Evidence for an antigenic determinant at the nucleotide-binding domain of the enzyme

Biochimica Et Biophysica Acta
I CarlbergB Mannervik

Abstract

Glutathione reductase has been purified to homogeneity by a method which is an improvement of an earlier procedure (Carlberg, I. and Mannervik, B. (1975) J. Biol. Chem. 250, 5475-5480). The new steps in the purification scheme include affinity chromatography on 2',5' ADP-Sepharose 4B. Antibodies to glutathione reductase from rat liver were raised in rabbits and used for analysis of the enzyme by quantitative 'rocket' immunoelectrophoresis. Glutathione reductase from human erythrocytes, porcine erythrocytes, and calf-liver gave precipitin lines showing partial identity with the rat liver enzyme in Ouchterlony double diffusion experiments. Enzyme from spinach, yeast (Saccharomyces cerevisiae), and the photosynthetic bacterium Rhodospirillum rubrum did not give precipitates with the antibodies to the enzyme from rat liver. Titration of glutathione reductase from the different sources with antibodies confirmed the cross-reactivity of the mammalian enzymes; the human enzyme giving the strongest heterologous reaction. No reaction was observed with the enzyme from spinach, yeast, and Rhodospirillum rubrum. NADPH, NADP+, and 2',5' ADP were found to inhibit the interaction between antibodies and glutathione reductase from rat liver and ...Continue Reading

References

Mar 1, 1976·Archives of Biochemistry and Biophysics·G MoroffK G Brandt
Jul 28, 1978·Biochemical and Biophysical Research Communications·V Boggaram, B Mannervik
Jun 15, 1977·Journal of Molecular Biology·H A ZappeG E Schulz
Jan 1, 1969·Archiv für Mikrobiologie·M Boll

❮ Previous
Next ❯

Citations

Jan 17, 2014·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·Kavita AroraArvind K Srivastava
Jan 1, 1993·The International Journal of Biochemistry·C García-AlfonsoJ López-Barea
Jan 1, 1985·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·K LarsenB Mannervik
Nov 26, 2010·Journal of Clinical Biochemistry and Nutrition·Yasumasa OkazakiShigeru Okada
Sep 11, 2012·Journal of Clinical Biochemistry and Nutrition·Yasumasa OkazakiShinya Toyokuni
May 30, 2008·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Bilge TekmanMehmet Ciftci
Jan 1, 1994·Experimental Lung Research·K A Kennedy, N L Lane
Apr 2, 2014·Research in Developmental Disabilities·Eduardo Benedetti ParisottoDanilo Wilhelm Filho
Nov 11, 2003·Preparative Biochemistry & Biotechnology·Mustafa EratMehmet Ciftci
Mar 23, 2018·Journal of Basic Microbiology·Priya FukeHemant J Purohit
Jan 4, 2019·Acta Crystallographica. Section F, Structural Biology Communications·Mwilye SikanyikaMegan J Maher

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.