PMID: 6979751May 1, 1982Paper

Purification and initial characterization of rat interleukin 2

Proceedings of the National Academy of Sciences of the United States of America
G Di Sabato

Abstract

With the sequential use of dialysis, chromatography on Sephadex G-100, reactive red 120-agarose, p-hydroxymercuribenzoate-agarose, phenyl-Sepharose, poly(L-lysine)-agarose, and isoelectrofocusing, the thymocyte stimulatory factor activity of interleukin 2 from rat spleen was purified about 8,000-fold. By the same procedures, the T cell growth factor activity of interleukin 2 was purified about 6,000-fold. The major peaks of thymocyte stimulatory factor activity and T cell growth factor activity cochromatographed in the various systems used, but T cell growth factor activity without significant thymocyte stimulatory factor activity was sometimes present. Both activities were found to have a pI between pH 5.50 and 6.30. Detectable thymocyte stimulatory factor activity was found at concentrations as low as 0.1-0.2 ng of protein per 0.2 ml. Dose--response plots were linear up to at least 1 ng of protein. Preparations of interleukin 2 labeled with 125I-containing Bolton--Hunter reagent and purified by the procedure mentioned above were electrophoresed on a polyacrylamide gel under denaturing and reducing conditions. The 125I-labeled material migrated in one major band with a molecular weight under 20,000 and three smaller bands with...Continue Reading

References

Mar 15, 1976·Cellular Immunology·D M Chen, G Di Sabato
Jan 1, 1980·Journal of Immunological Methods·A Altman, D H Katz
Jan 1, 1980·Annals of the New York Academy of Sciences·M EvingerS Pestka
Sep 1, 1980·Cellular Immunology·M Altin, G Di Sabato
Oct 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·J W Mier, R C Gallo
May 1, 1980·Molecular Immunology·K A SmithF W Ruscetti

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Citations

Jan 1, 1986·Molecular Biology Reports·W DegraveW Fiers
Jun 1, 1988·Cellular Immunology·L P Thompson, G Di Sabato
Jan 1, 1985·Developmental and Comparative Immunology·D Watkins, N Cohen
Jan 1, 1990·Developmental and Comparative Immunology·C Corbel, J L Thomas
Apr 1, 1984·Molecular Immunology·S M BellP L Stern
Sep 1, 1984·Molecular Immunology·T I ValyakinaV A Nesmeyanov
Nov 1, 1995·Brain Research. Brain Research Reviews·U K Hanisch, R Quirion
May 1, 1985·Veterinary Immunology and Immunopathology·L S EnglishS T Licence
Oct 1, 1992·Veterinary Immunology and Immunopathology·T J MyersR H Fetterer
Jan 1, 1984·Immunology Letters·V A NesmeyanovV A Kulshin
Jan 1, 1984·Immunology Letters·T Stanton, M Maynard
Feb 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·A S SternS Gillis
Apr 1, 1983·Kidney International·L M Patt, J C Houck
Jul 1, 1983·European Journal of Immunology·M SchnetzlerR M Franklin

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