PMID: 8948495Nov 14, 1996Paper

Purification and kinetic properties of a D-amino-acid peptide hydrolyzing enzyme from pig kidney cortex and its tentative identification with renal membrane dipeptidase

Biochimica Et Biophysica Acta
T WatanabeR H Yamada

Abstract

We previously reported the presence of an enzyme activity which hydrolyzes Gly-D-Asp in pig kidney cortex. In the present study, an enzyme which hydrolyzes this peptide and other peptides having a low number of D- and L-amino acids has been purified from the brush border membranes of the same tissue. The native enzyme, having a molecular weight of 99,000, was apparently a homodimer of a subunit with a molecular weight of 48,000 and its optimum pH was 7.8 with Gly-D-Ala as substrate. The enzyme hydrolyzed many dipeptides, but not most of the tripeptides tested with a few exceptions, from which the carboxyl-terminal amino acid was liberated by the enzyme. Of the dipeptides examined, those having a D-amino acid at the amino-terminal were poor substrates, whereas those bearing a D-amino acid at the carboxyl-terminal were good substrates, comparable with their diastereomers with a L-amino acid at the same position. The enzyme was potently inhibited by cilastatin but not by amastatin and bestatin. Metal ion chelators and dithiothreitol were also inhibitory. Comparison of the properties of present enzyme with those of other known enzymes suggests that this should be tentatively identified with renal membrane dipeptidase. The demonstra...Continue Reading

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Citations

Jan 27, 2004·Biochemical and Biophysical Research Communications·Tadatoshi KinouchiYasuo Kagawa
Jul 30, 1999·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Y KeraR H Yamada
Oct 20, 2005·Journal of Bioscience and Bioengineering·Y Asano, T L Lübbehüsen
Mar 4, 2006·Preparative Biochemistry & Biotechnology·Marina KrstanovićFabio Benedetti
Nov 15, 2006·Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]·Ryo-hei YamadaShouji Takahashi
Jan 17, 2020·Critical Reviews in Toxicology·Patrick E Hanna, M W Anders
Jun 17, 1999·Annals of Clinical Biochemistry·Y FukumuraM Yabuuchi

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