Purification and kinetics of a protease-resistant, neutral, and thermostable phytase from Bacillus subtilis subsp. subtilis JJBS250 ameliorating food nutrition

Preparative Biochemistry & Biotechnology
Jinender JainBijender Singh

Abstract

A novel protease-resistant and thermostable phytase from Bacillus subtilis subsp. subtilis JJBS250 was purified 36-fold to homogeneity with a combination of ammonium sulfate precipitation followed by Q-Sepharose and Sephadex G-50 chromatographic techniques. The estimated molecular mass of the purified phytase was 46 kDa by electrophoresis with optimal activity at pH 7.0 and 70 °C. About 19% of original activity was maintained at 80 °C for 10 min. Phytase activity was stimulated in presence of surfactants like Tween-20, Tween-80, and Triton X-100 and metal ions like Ca+2, K+, and Co+2 and it was inhibited by SDS and Mg+2, Al+2, and Fe+2. Purified enzyme showed specificity to different salts of phytic acid and values of Km and Vmax were 0.293 mM and 11.49 nmoles s-1, respectively for sodium phytate. The purified enzyme was resistant to proteases (trypsin and pepsin) that resulted in amelioration of food nutrition with simultaneous release of inorganic phosphate, reducing sugars, and soluble protein.

References

Oct 12, 2001·Journal of Protein Chemistry·Y M ChoiJ M Kim
Apr 16, 2003·Critical Reviews in Biotechnology·Ashima Vohra, T Satyanarayana
May 28, 2004·Journal of Biotechnology·Anne Casey, Gary Walsh
Jun 23, 2005·Current Microbiology·Jaiesoon ChoYunjaie Choi
Sep 13, 2006·Journal of Industrial Microbiology & Biotechnology·H K GulatiH S Saini
Dec 5, 2008·Bioresource Technology·Bijender Singh, T Satyanarayana
Jan 17, 2012·Journal of Bioscience and Bioengineering·Lotis Escobin-MoperaKozo Asano
Apr 11, 2012·American Journal of Health Behavior·Donna M Anderson, Karen M Skemp
Nov 16, 2012·Journal of Agricultural and Food Chemistry·Juan Mario Sanz-PenellaMonika Haros
Oct 25, 2013·Preparative Biochemistry & Biotechnology·Feyisola O FasimoyeMorakinyo D Sanni
Aug 19, 2014·Journal of Animal Physiology and Animal Nutrition·B Singh, T Satyanarayana
Jan 16, 2015·Applied Biochemistry and Biotechnology·Chinreddy Subramanyam ReddyMalireddy K Reddy

❮ Previous
Next ❯

Citations

Sep 17, 2020·Journal of Industrial Microbiology & Biotechnology·Yuankun HelianDawei Zhang

❮ Previous
Next ❯

Methods Mentioned

BETA
gel filtration
electrophoresis
ion exchange chromatographic

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.