Purification and mass spectrometry identification of leukotriene D4 synthesized by human alveolar macrophages

Biochemical and Biophysical Research Communications
M DamonA Crastes de Paulet

Abstract

Human AM obtained by BAL from normal subjects and asthmatic patients converted [1-14C]-AA into a polar labeled metabolite. The structure of this metabolite, after two successive purifications on TLC (silicagel plates then reversed phase plates) and mass spectrometric analysis was shown to be identical to an authentic sample of LTD4. The amount of LTD4 recovered in the culture medium of AM was attempted to be related to pathological lung profile. In our experimental conditions AM from allergic asthmatics synthetized more LTD4 than cells from healthy subjects and from aspirin sensitive asthmatic patients.

References

Apr 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·L OrningB Samuelsson
Jun 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·R A LewisE J Corey

❮ Previous
Next ❯

Citations

May 1, 1986·Veterinary Research Communications·P A HenricksF P Nijkamp
Sep 20, 1983·Biochimica Et Biophysica Acta·J T DuP W Ramwell
Apr 15, 1988·Biochimica Et Biophysica Acta·M Abe, T E Hugli
Jun 7, 2005·Burns : Journal of the International Society for Burn Injuries·Göksel SenerBerrak C Yeğen
May 14, 2005·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Levent KabasakalBerrak C Yeğen
Jul 1, 1987·The American Review of Respiratory Disease·J G GarciaA B Malik
Nov 1, 1988·The American Review of Respiratory Disease·M S BalterM Peters-Golden
Feb 1, 1990·The American Review of Respiratory Disease·Y Sibille, H Y Reynolds
Jan 1, 1986·The Journal of Clinical Investigation·M LavioletteP Borgeat
Jun 1, 1990·Prostaglandins, Leukotrienes, and Essential Fatty Acids·M AbeN Shigematsu
May 26, 2015·International Braz J Urol : Official Journal of the Brazilian Society of Urology·Alper OtunctemurNurver Ozbay
Mar 12, 1984·FEBS Letters·J VerhagenJ F Vliegenthart
Sep 27, 2007·Journal of Pharmacological Sciences·Gunnur Ozbakis DengizHalis Suleyman
Nov 1, 1988·Immunological Investigations·G Marone
Jun 1, 1988·The American Journal of Physiology·G P BrownG W Hunninghake
Jan 1, 1987·Clinical Immunology and Immunopathology·P R BachwichS L Kunkel
Jan 1, 1989·Clinical Immunology and Immunopathology·G MaroneA Genovese
Oct 1, 1990·Prostaglandins, Leukotrienes, and Essential Fatty Acids·H C LeeN Mori
Dec 14, 2004·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Göksel SenerGül Ayanoğlu-Dülger
Sep 1, 1985·Annales De L'Institut Pasteur. Immunology·P BraquetM Braquet

❮ Previous
Next ❯

Related Concepts

Related Feeds

Asthma

This feed focuses in Asthma in which your airways narrow and swell. This can make breathing difficult and trigger coughing, wheezing and shortness of breath.

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.