Purification and partial amino acid sequences of two distinct albumins from turtle plasma

Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
M A BrownP Licht

Abstract

Two putative albumins, denoted Alb-1 (apparent molecular mass of 67 kDa) and Alb-2 (68 kDa), were purified from plasma of the emydid turtle (Trachemys scripta). Concentrations in serum or plasma were determined by radioimmunoassay using 125I-labeled Alb-1. In juvenile turtles (less than 2 years of age), serum concentrations of Alb-1 and Alb-2 were 2.72 +/- 0.23 mg/ml and 1.68 +/- 0.22 mg/ml, respectively, while concentrations in plasma pooled from adult turtles were 4.2 mg/ml and 2.6 mg/ml, respectively. The two albumins are immunologically distinct from one another as determined by both radioimmunoassay with 125I-labeled Alb-1 and Western blot analysis with antichicken albumin antiserum. Determination of the amino acid compositions of Alb-1 and Alb-2, and of albumin purified from plasma of the common snapping turtle (Chelydra serpentina), suggested that Alb-1 is more similar to albumins of other animals than is Alb-2. This was also indicated by Western blot analysis and by determining the N-terminal amino acid sequences of Alb-1 (40 residues) and Alb-2 (15 residues). Thus, it appears that two distinct forms of albumin are synthesized by T. scripta, possibly as a result of gene duplication and divergence.

References

Jan 1, 1977·Annual Review of Biochemistry·A C WilsonT J White
Mar 18, 1976·Biochimica Et Biophysica Acta·I A MacLaren, M L Petras
Jan 15, 1975·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·H C Dessauer, F H Pough
Feb 1, 1992·Protein Science : a Publication of the Protein Society·J E Gray, R F Doolittle
Oct 1, 1990·Molecular Endocrinology·V Averyhart-Fullard, R C Jaffe
Nov 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·J MadisonF W Putnam
May 1, 1989·Molecular Endocrinology·D R SchoenbergR L Pastori
Jan 1, 1987·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·M Hernández De MoralesC Baerga-Santini
Jan 1, 1985·Advances in Protein Chemistry·T Peters
Jan 1, 1985·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·M S Kaldy, C L Darcel
Dec 29, 1972·Journal of Molecular Evolution·D G Wallace, A C Wilson
Aug 31, 1972·Biochimica Et Biophysica Acta·H NaganoR Shukuya
Apr 1, 1968·Comparative Biochemistry and Physiology·R J Masat, H C Dessauer
Aug 11, 1981·Nucleic Acids Research·B WestleyR Weber
Jan 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·C W HeizmanA Olomucki
Apr 1, 1995·Biochemical Genetics·S B Kuznetsov
Mar 1, 1995·General and Comparative Endocrinology·K W Selcer, B D Palmer
Feb 15, 1994·Archives of Biochemistry and Biophysics·P Licht, M F Moore
Jan 1, 1994·Advances in Protein Chemistry·D C Carter, J X Ho
Jan 1, 1993·General and Comparative Endocrinology·K A Glennemeier, P Licht
Mar 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·S WatkinsF W Putnam
Apr 1, 1993·General and Comparative Endocrinology·K A Glennemeier, P Licht
Apr 1, 1996·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·M A BrownG K Chambers
Jun 1, 1997·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·M A BrownG K Chambers
Mar 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·S B HedgesL R Maxson
Dec 1, 1963·Proceedings of the Society for Experimental Biology and Medicine·C STORMONT, Y SUZUKI

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Citations

May 20, 2008·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·M P IkonomopoulouA J Bradley
Apr 6, 2007·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Victoria J MetcalfStephen O Brennan
Jan 13, 2020·Journal of Zoo and Wildlife Medicine : Official Publication of the American Association of Zoo Veterinarians·Dana Franzen-KleinMatthew C Allender
Mar 5, 2004·Environmental Science & Technology·Corina M Ciocan, Jeanette M Rotchell

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