Purification and Partial Characterization of a Novel Bacteriocin Synthesized by Lactobacillus paracasei HD1-7 Isolated from Chinese Sauerkraut Juice

Scientific Reports
Jingping GeWenxiang Ping

Abstract

Bacteriocins have antimicrobial activities against food-spoiling bacteria and food-borne pathogens. Paracin 1.7, a bacteriocin synthesized by Lactobacillus paracasei HD1-7 isolated from Chinese sauerkraut juice, was studied. Following partial purification with ammonium sulfate precipitation, CM Sepharose Fast Flow, and Sephadex G-10 chromatography, the molecular weight of Paracin 1.7 was about 10 kDa based on Tricine-SDS-PAGE results. A 2.87 fold purified bacteriocin was produced, reaching a final yield of 39.93% and the specific activity of 1.56 × 10(3) AU/mg. The N-terminal amino acid sequence of Paracin 1.7 was VSNTFFA, and the LC/LTQ results revealed that the N-terminal amino acid sequence was similar to that of ABC-type oligopeptide transport system protein and N-acetylmuramoyl-L-alanine amidase. Paracin 1.7 was sensitive to protease K, had antimicrobial activities at a broad pH range (3.0-8.0), and was heat resistant (121 °C for 20 min). Paracin 1.7 from Lactobacillus paracasei HD1-7 is a novel bacteriocin that has potential applications in food preservation.

References

Mar 1, 1993·European Journal of Biochemistry·M J GarceráW N Konings
Oct 3, 2000·Natural Product Reports·M J van Belkum, M E Stiles
Aug 2, 2005·Journal of Peptide Science : an Official Publication of the European Peptide Society·Gunnar FimlandJon Nissen-Meyer
Oct 6, 2005·Nature Reviews. Microbiology·Paul D CotterR Paul Ross
Jun 9, 2006·Microbiology and Molecular Biology Reviews : MMBR·Djamel DriderHervé Prévost
Sep 11, 2007·Journal of Molecular Microbiology and Biotechnology·Camilla OppegårdJon Nissen-Meyer
Jan 12, 2008·Biochimica Et Biophysica Acta·Per RognePer Eugen Kristiansen
Apr 13, 2010·Probiotics and Antimicrobial Proteins·Jon Nissen-MeyerPer Eugen Kristiansen

❮ Previous
Next ❯

Citations

Jun 10, 2017·Archives of Microbiology·Shih-Chun YangJia-You Fang
Jan 11, 2017·Applied Microbiology and Biotechnology·Vilma KaškonienėAudrius Maruška
Jul 1, 2019·Comprehensive Reviews in Food Science and Food Safety·María G Venegas-OrtegaGuadalupe V Nevárez-Moorillón
Jul 15, 2021·International Journal of Microbiology·Ellen TwomeyMáire Begley
Aug 29, 2021·Applied Microbiology and Biotechnology·Yuanyuan ZhuQing Gu
Oct 16, 2021·Biotechnology Letters·Gulyaim K AbitayevaSerik M Shaikhin
Sep 24, 2021·Protein and Peptide Letters·Sreekala S, Usha Devi Muraleedharan

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis
amino acid sequencing

Related Concepts

Related Feeds

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

ABC Transporters & Multidrug Resistance

ABC Transporters or ATP-Binding Cassette Transporters are responsible for pumping out antibiotics in the cell and can lead to multidrug resistance in bacteria. Discover the latest research on ABC Transporters & Multidrug Resistance here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.