PMID: 376312Apr 1, 1979Paper

Purification and partial characterization of a factor, a mating hormone produced by mating-type-a cells from Saccharomyces cerevisiae

European Journal of Biochemistry
R Betz, W Duntze

Abstract

Cells of Saccharomyces cerevisiae exhibiting the a mating type secrete into the culture medium a mating-type-specific hormone activity (a factor), which specifically causes a transient arrest of DNA replication and cell division in cells of the opposite mating type, alpha. Three compounds exhibiting a factor activity have been found in culture filtrates from a cells. The most active compound has been purified more than 10(5)-fold and appears to be homogeneous on the basis of thin-layer chromatography and thin-layer electrophoresis in different systems. We propose that this compound, which exhibits in alpha cells the biological activities that have been attributed to a factor, represents pure a factor. a factor has been characterized as a very hydrophobic undecapeptide with the following amino acid composition: H2N-Tyr (Asx1, Gly1, Ala1, Val1, Ile2, Phe1, Lys1, Trp1, Pro1). Although in their respective target cells the biological effects of a factor and of alpha factor, the corresponding mating hormone of mating-type-alpha cells, are remarkably similar, the primary structures of both hormones appear to be quite different.

References

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Citations

Apr 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·H Liao, J Thorner
Jan 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·V L MacKayM L Parker
Nov 1, 1996·The Journal of Cell Biology·L Elia, L Marsh
Feb 1, 1979·The Journal of Cell Biology·J S Tkacz, V L MacKay
Aug 31, 2012·Microbiology and Molecular Biology Reviews : MMBR·Susan Michaelis, Jemima Barrowman
Oct 11, 2008·PLoS Computational Biology·Marcelo BeharTimothy C Elston
Mar 20, 2010·PLoS Computational Biology·Steven S AndrewsAdam P Arkin
Jan 1, 1986·CRC Critical Reviews in Biochemistry·F Naider, J M Becker
Feb 22, 2018·Proteomics·Paige E Erpf, James A Fraser
Oct 6, 1983·Nature·J R Strazdis, V L MacKay
May 1, 1991·Yeast·J G De Nobel, J A Barnett
Oct 15, 2013·Genetics·Lori B Huberman, Andrew W Murray
May 18, 2007·FEMS Yeast Research·Laura Ongay-LariosRoberto Coria
Oct 8, 2014·Antimicrobial Agents and Chemotherapy·Vibhati V KulkarnySamuel A Lee
Mar 1, 1988·Molecular and Cellular Biology·S Michaelis, I Herskowitz

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