Purification and partial characterization of a putative precursor to staphylococcal enterotoxin B.

Infection and Immunity
R K Tweten, J J Iandolo

Abstract

A putative precursor to staphylococcal enterotoxin B (SEB) has been identified as a component of purified membranes from Staphylococcus aureus S6. Agarose gel immunodiffusion analysis of the solubilized membranes demonstrated an immunoreactive protein that formed complete lines of identity with purified extracellular SEB. This putative precursor (pSEB) also had a different electrophoretic mobility from that of extracellular SEB when analyzed by immunoelectrophoresis. When membrane proteins from S6 were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and then transferred to nitrocellulose sheets and probed with I-125 labeled, affinity-purified anti-SEB, the pSEB band was identified. The pSEB was approximately 3,500 daltons larger than extracellular SEB. This component was purified by immunoprecipitation and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Two-dimensional peptide maps of the putative SEB precursor revealed that most of the tryptic peptides were identical to those of mature extracellular SEB. When purified membranes of other SEB+ (DU4916 and 10-275) and SEB- (RN450, RN451, S6R, and FR1100) S. aureus strains were analyzed by the nitrocellulose blot procedure, only the SEB+ strains co...Continue Reading

References

Jan 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·R D PalmiterK A Walsh
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·A W StraussA W Alberts
Apr 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·H Inouye, J Beckwith
Aug 8, 1977·Biochemical and Biophysical Research Communications·J SekizawaM Inouye
Mar 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·L L RandallL G Josefsson
Jun 1, 1979·European Journal of Biochemistry·G von Heijne, C Blomberg
Sep 1, 1976·Canadian Journal of Microbiology·R D Miller, D Y Fung
Aug 1, 1971·Infection and Immunity·A W Jarvis, R C Lawrence
Jul 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·D Shields
Jan 11, 1980·Nucleic Acids Research·B BowenH Weintraub
Feb 12, 1980·Biochemical and Biophysical Research Communications·F GossardM Chrétien
Jul 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·A S LewinG Schatz
Feb 27, 1980·Biochimica Et Biophysica Acta·J L MiddlebrookP Argos

❮ Previous
Next ❯

Citations

Feb 1, 1992·Zentralblatt für Veterinärmedizin. Reihe B. Journal of veterinary medicine. Series B·F Javier HernándezG Suárez
Jul 1, 1992·FEMS Microbiology Immunology·J GoyacheE Gómez-Lucía
Jan 1, 1990·Critical Reviews in Microbiology·G A BohachP M Schlievert
Sep 1, 1985·Applied and Environmental Microbiology·K K ChristiansonJ J Iandolo
Jan 1, 1983·Journal of Bacteriology·R K Tweten, J J Iandolo
Nov 1, 1983·Journal of Bacteriology·R K TwetenJ J Iandolo
Jan 1, 1985·Infection and Immunity·K Y Lee, T H Birkbeck
May 1, 1989·Journal of Clinical Microbiology·M P StricklerP Gemski
Jul 1, 1988·Infection and Immunity·J Meador, R K Tweten
Sep 1, 1988·Journal of Bacteriology·M B Johns, S A Khan
Apr 1, 1986·Journal of Bacteriology·C L Jones, S A Khan
May 1, 1986·Journal of Bacteriology·M P Jackson, J J Iandolo

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.