Purification and partial characterization of an agglutinin from Octopus maya serum

Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
Juan AlpucheEdgar Zenteno

Abstract

A 66-kDa lectin (OmA) was purified from the serum of the Yucatan peninsula endemic octopus (Octopus maya) by a single step affinity chromatography on glutaraldehyde-fixed stroma from rat erythrocytes. OmA corresponds to 0.8% of the total circulating protein in the hemolymph; it is composed of three equal subunits of 22kDa each, and 7.4% of linked carbohydrates. The amino acids' composition indicated that agglutinin contained mainly aspartic and glutamic acids, and cysteine and methionine were identified in minor proportion. OmA agglutinates mainly rat, guinea pig, and rabbit erythrocytes, and this activity is partially inhibited by galactosamine, melobiose, galacturonic acid, mannose, and methyl alpha and beta galactosides. Hemagglutinating activity is not dependent on divalent cations, such as Ca(2+), Mg(2+), or Mn(2+). The OmA subunits showed no identity for any lectin in databases but partial identity with the type A hemocyanin from Octopus dolfleini hemolymph; the main similarities are related to tyrosinase domains and copper A and B sites that conform to the oxygen-binding site of hemocyanin.

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Citations

May 13, 2014·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Charles Le PabicNoussithé Koueta
Feb 4, 2014·Developmental and Comparative Immunology·Christopher J Coates, Jacqueline Nairn
Jan 3, 2013·Journal of Molecular Recognition : JMR·Rômulo Farias CarneiroAlexandre Holanda Sampaio
Sep 5, 2012·Fundamental & Clinical Pharmacology·Raniere da M MouraBenildo S Cavada
May 20, 2015·Fish & Shellfish Immunology·C Gestal, S Castellanos-Martínez
Jun 29, 2015·Fish & Shellfish Immunology·Maria G CastilloNina R Joffe
Jul 30, 2014·Fish & Shellfish Immunology·Charles Le PabicAntoine Serpentini
Oct 14, 2016·Frontiers in Physiology·Anna Di Cosmo, Gianluca Polese
Oct 30, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sergio Espíndola MateosMargarito Martínez-Cruz
Jan 16, 2021·Carbohydrate Research·Jeyachandran SivakamavalliBaskaralingam Vaseeharan
Nov 13, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sergio Espíndola MateosMargarito Martínez-Cruz

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