PMID: 2495273Apr 1, 1989Paper

Purification and partial characterization of the glycine decarboxylase multienzyme complex from Eubacterium acidaminophilum

Journal of Bacteriology
W Freudenberg, J R Andreesen

Abstract

The proteins P1, P2, and P4 of the glycine cleavage system have been purified from the anaerobic, glycine-utilizing bacterium Eubacterium acidaminophilum. By gel filtration, these proteins were determined to have Mrs of 225,000, 15,500, and 49,000, respectively. By sodium dodecyl sulfate-polyacrylamide gel electrophoresis, protein P1 was determined to have two subunits with Mrs of 59,500 and 54,100, indicating an alpha 2 beta 2 tetramer, whereas the proteins P2 and P4 showed only single bands with estimated Mrs of 15,500 and 42,000, respectively. In reconstitution assays, proteins P1, P2, P4 and the previously reported lipoamide dehydrogenase (P3) had to be present to achieve glycine decarboxylase or synthase activity. All four glycine decarboxylase proteins exhibited highest activities when NADP+ was used as the electron acceptor or when NADPH was used as the electron donor in the glycine synthase reaction. The oxidation of glycine depended on the presence of tetrahydrofolate, dithioerythreitol, NAD(P)+, and pyridoxal phosphate. The latter was loosely bound to the purified protein P1, which was able to catalyze the glycine-bicarbonate exchange reaction only in combination with protein P2. Protein P2 could not be replaced by li...Continue Reading

References

Oct 1, 1978·Analytical Biochemistry·G F MiozzariR Hütter
Feb 1, 1984·Archives of Biochemistry and Biophysics·J R Sokatch, G Burns
Jun 25, 1982·Molecular and Cellular Biochemistry·G Kikuchi, K Hiraga
Apr 1, 1983·Journal of Bacteriology·P Dürre, J R Andreesen

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Citations

Sep 1, 1993·European Journal of Biochemistry·K Okamura-IkedaY Motokawa
Jan 1, 1994·Antonie van Leeuwenhoek·J R Andreesen
Oct 23, 2008·Proceedings of the Japan Academy. Series B, Physical and Biological Sciences·Goro KikuchiKoichi Hiraga
Feb 4, 2010·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Dirk HasseHermann Bauwe
Jul 28, 2016·Frontiers in Microbiology·Frank R BengelsdorfPeter Dürre
Apr 21, 2020·The Plant Journal : for Cell and Molecular Biology·Maria WittmißHermann Bauwe
Dec 1, 2004·EcoSal Plus·George V Stauffer

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