Purification and partial characterization of hyaluronate lyase (EC from Propionibacterium acnes

Journal of General Microbiology
E InghamW J Cunliffe


Hyaluronidase from Propionibacterium acnes has been purified 13,000-fold from the culture supernatant to homogeneity (as determined by polyacrylamide disc gel electrophoresis). The molecular weight of the purified enzyme was 85,110 as determined by gel filtration. The purified enzyme had a pH optimum at 6.4, was stable between pH 5 and 5.8 and was completely inactivated after 15 min at 50 degrees C. Preliminary studies suggested that the enzyme is active against chondroitin 4- and 6-sulphates, but not against dermatan sulphate. Analysis by paper chromatography of the reaction products from the degradation of hyaluronic acid by bacterial, testicular and P. acnes enzymes suggested that the P. acnes enzyme is similar in its mode of action to other bacterial hyaluronate lyases. The enzyme from P. acnes may thus be tentatively classified as a hyaluronate lyase.


Aug 31, 2010·BMC Microbiology·Carsten HollandHolger Brüggemann
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Related Concepts

Chondroitin Sulfate, Zinc Salt
Dermatan Sulfate
Hydrogen-Ion Concentration
Propionibacterium acnes

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