PMID: 9528655Apr 7, 1998Paper

Purification and preliminary characterisation of praelongin phospholipases, antiplatelet agents from the snake venom of Acanthophis praelongus

Biochimica Et Biophysica Acta
K L Sim

Abstract

Three praelongin phospholipases were chromatographically purified from the snake venom of Acanthophis praelongus. The purity and homogeneity of the praelongins were assessed by RP-HPLC, HPCE and mass spectrometry. The purified enzymes, praelongins 2bIII, 2cII and 2cIV were found to have phospholipase A2 activities with specific activities of 31.4 +/- 0.4, 326.1 +/- 10.2 and 362.5 +/- 12.0 U/mg, respectively. Mass spectrometry studies showed the molecular mass of praelongin 2bIII to be 12,782.9 +/- 2.6 and praelongins 2cII and 2cIV to have very similar molecular mass values, 12,971.4 +/- 4.5 and 12,971.9 +/- 3.6, respectively. However, platelet aggregation studies showed the praelongins to display different IC50 values, 180 microM for praelongin 2cII and 55 microM for praelongin 2cIV; praelongin 2bIII was found to be a more potent antiplatelet agent, having an IC50 of 0.65 microM. Praelongins 2bIII, 2cIV and 2cII were found to have pI values of 10.3 +/- 0.3, 9.6 +/- 0.6 and 9.4 +/- 0.6 as determined by HPCE. The antiplatelet potencies do not correspond to their in vitro phospholipase catalytic potencies, but appear to be related to the enzyme isoelectric points.

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Citations

May 9, 2001·Biochimica Et Biophysica Acta·R GaoP Gopalakrishnakone
Oct 3, 2012·PLoS Neglected Tropical Diseases·Christopher I JohnstonUNKNOWN ASP Investigators
Sep 1, 1999·Haemophilia : the Official Journal of the World Federation of Hemophilia·I Scharrer
Oct 2, 2007·Toxicon : Official Journal of the International Society on Toxinology·J SteutenB G Fry
Apr 25, 2006·Blood Cells, Molecules & Diseases·Xiao-Yan DuYun Zhang
Aug 22, 2006·Toxicon : Official Journal of the International Society on Toxinology·Wayne C Hodgson, Janith C Wickramaratna
May 30, 2003·Toxicological Sciences : an Official Journal of the Society of Toxicology·Janith C WickramaratnaWayne C Hodgson
Nov 9, 2001·Electrophoresis·R Weinberger
Mar 10, 2006·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·David E GerberMichael B Streiff
May 10, 2012·Zhurnal evoliutsionnoĭ biokhimii i fiziologii·Ia A al'tman

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