Purification and preliminary characterization of a xylanase from Thermomyces lanuginosus strain SS-8.

3 Biotech
Smriti ShrivastavaKunal Mukhopadhyay

Abstract

Thermomyces lanuginosus SS-8 was isolated from soil samples that had been collected from near self-heating plant material and its extracellular cellulase-free xylanase purified approximately 160-fold using ion exchange chromatography and continuous elution electrophoresis. This xylanase was thermoactive (optimum temperature 60 °C) at pH 6.0 and had a molecular weight of 23.79 kDa as indicated by SDS-PAGE electrophoresis. The xylanase rapidly hydrolyzed xylan directly to xylose without the production of intermediary xylo-oligosaccharides within 15 min of incubation under optimum conditions. This trait of rapidly degrading xylan to xylose as a sole end-product could have biotechnological potential in degradation of agro-wastes for bioethanol manufacturing industry.

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Citations

Oct 24, 2019·Applied Microbiology and Biotechnology· Alokika, Bijender Singh

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Datasets Mentioned

BETA
GQ469970

Methods Mentioned

BETA
electrophoresis
Assay

Software Mentioned

ImageQuant TL
BLASTn
ImageQuant

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